Fig 1.
Reaction pathway for synthesis acetohydrazide derivative of CZT and its coupling with proteins (A) and the coupling reaction mechanism (B). Protein-COOH is the BSA and KLH, EDC is 1-ethyl-3-(3-dimethyl-aminopropyl carbodiimide hydrochloride and NHS is N-hydroxysuccinimide.
Table 1.
1H-NMR spectral data of acetohydrazide derivative of CZT.
Table 2.
13C-NMR spectral data of acetohydrazide derivative of CZT.
Fig 2.
Panel (A): affinity of antisera from mice (1–4) for CZT, panel (B): checkerboard titration for CZT-BSA versus anti-CZT antibody. Varying concentrations of CZT-BSA conjugate (0.15 − 5 μg/mL) were coated onto the microwell plates and varying dilutions of the anti-CZT antibody were allowed to bind to the coated CZT-BSA. Dilutions of anti-CZT antibody were 20 (), 40 (
), 80 (
), 160 (
) folds. Panel (C): calibration curve (
) and precision profile (
) of the proposed ELISA for CZT.
Fig 3.
Schematic diagram for the proposed ELISA for CZT.
Table 3.
Summary for the optimum conditions of ELISA for CZT.
Table 4.
Precisions of the proposed ELISA for CZT at different concentration levels.
Table 5.
Analytical recovery of CZT spiked in plasma samples.
Table 6.
Comparison of ELISA with liquid chromatographic techniques for analysis of CZT in human plasma.