Table 1.
Comparison of Axiom Microbiome Array vs 16S rRNA sequencing vs metagenomic sequencing.
Table 2.
Probe summary for Axiom Microbiome Array.
The number of microbial sequences were current as of October 2014.
Table 3.
Analysis of the sensitivity of bacterial, fungal and viral target detection on the Axiom Microbiome Array.
The genomic DNA was spiked into soil DNA background. The average number of probes detected was an average of two replicates with standard deviation.
Table 4.
Axiom Microbiome Array testing results of ATCC Metagenomic Control Material (ATCC MSA-4000).
Fig 1.
The log of conditional scores vs. ratio of conditional/initial scores graph of the ATCC Metagenomic Control Material.
The x-axis (Log of Conditional Scores) provides information on the relative contribution of each target to the model provided by Axiom MiDAS, while the y-axis provides the ratio of the actual probe-level data used to contribute to the model relative to its possible maximal contribution. The default threshold is 0.2. Each circle represents one unique target sequence. The circle highlighted represented Klebsiella pneumoniae subsp. pneumoniae MGH 78578 plasmid pKPN. All other genomic targets were detected at a ratio of >0.6.
Fig 2.
The Pseudomonas aeruginosa probes detected from the ATCC Metagenomic Control Material across its genome.
This graph is plotted as the log intensity of probes detected for a given target versus the position of the probe on the target sequence. Only probes that are above 99% of background probes (in round dots) are considered positive. The probes that are between 95% and 99% (in triangle) and below 95% (square) are not considered positive.
Table 5.
Axiom Microbiome Array results of ATCC 20 Strain Staggered Mix Genomic Material (ATCC MSA-1003).
Table 6.
Microbial species detected by Axiom Microbiome Array from a pig fecal sample (No. 289).
The pig was experimentally infected with PRRSV and PCV-2. Species are listed in decreasing order of conditional score.
Table 7.
Consensus of species level detection on the Axiom Microbiome Array from three technical replicates of 8 pig fecal samples.
Table 8.
Viruses detected from pig serum and tonsil samples on the Axiom Microbiome Array and comparison to the Agilent version of the LLMDA [18].
The results for the Agilent array were generated using total nucleic acid. The results from Axiom Microbiome Array were generated using RNA from these samples.