Fig 1.
Summary of the workflow and tools used to carry out the present study.
A schematic of the workflow devised to identify, model, and characterize various orthologs of the human eicosanoid pathway.
Table 1.
Summary of D. melanogaster eicosanoid synthesis enzymes candidates.
Fig 2.
A multiple sequence alignment of the D. melanogaster candidate CG4009 with human PTGS1 and known or high confidence predicted orthologs.
Red asterisks below the MSA denotes conserved key catalytic residues. The green asterisk marks the putative analog for the tyrosine. The blue asterisk denotes an insertion of 42 residues in CG4009 and reads X in the alignment.
Fig 3.
A theoretical eicosanoid synthesis pathway in D. melanogaster.
A graphical overview of fly candidates in the putative pathway shown alongside the human enzymes as well as superposed with it. Boxed names represent the intermediates or end products (Red: Prostaglandins; Blue: Thromboxanes; Green: Leukotrienes; Yellow: HETEs). Enzymes for which orthologs could not be identified are represented by dashed ovals. The three candidate groups are marked next to the enzymes in a similar manner as Table 1: high-confidence (regular font; Group 1), mid-range (underlined; Group 2), and distant (italicized and bold font; Group 3).