Table 1.
Leaf reaction of tested plants to 17 different BB isolates.
Fig 1.
Disease reaction of a population of 451 F2 plants and parents to BB race K3a.
a is distribution of lesion length of the F2 mapping population and parents at 14 DAI. b is resistant phenotype of parents and F1 plant. P1 is the resistant donor and P2 is susceptible donor.
Fig 2.
R-gene mapping by association and QTL analysis.
a is Manhattan plot produced by GWAS analysis with genome association and prediction integrated tool (GAPIT). The x-axis indicates genomic position of each SNP and the y-axis is negative logarithm of p-value, obtained from GWAS model. Large peaks on chromosome 11 suggest that the surrounding genomic region has a strong association with the BB race, K3a (p < 0.05) and QTNs marked with grey vertical dotted line. b is LOD profile and additive mapping using inclusive composite interval mapping (ICM) on whole rice chromosome. The SNP location was positioned as red circle on the position in the whole genome. Positive additive effects were derived from susceptible parent Ilpum and negative values were related to the resistant parent. The horizontal grey line is the LOD threshold calculated by 1,000 permutations and the vertical dotted lines indicated by the grey arrow show the loci from both analysis is identical on the chromosome 11.
Table 2.
Distribution of polymorphic SNPs across 12 rice chromosomes from parental survey.
Table 3.
Putative QTLs associated with BB resistance gene detected by composite and interval mapping.
Fig 3.
a is physical position of flanking markers and known BB R-genes on the chromosome 11 based on the Nipponbare genome. b is the position of the novel R-gene anchored by two DNA markers IBb27os11_14 and S_BB11.ssr_9. The number of recombinant events detected in the BC2F2 individuals were marked in brackets. c is gene locus listed on RAP-DB (IRGSP-1.0) based on the fine mapping. Nine candidates for the BB R-gene were included in approximately 119-Kbp.
Fig 4.
Gene expression of Os11g0687700 (a) and Os11g0688000 (b) by qRT-PCR of P8 and Ilpum after BB inoculation. mRNA expression levels of Os11g0688000 was analyzed at five time-points (0, 1, 2, 4, and 24h) after inoculation.