Table 1.
The occurrence of bacterial species on the cuticle of Galleria mellonella larvae reared on natural and semi-artificial food.
Fig 1.
Effects of fungal infection on G. mellonella larvae maintained on semi-artificial (A) and natural (B) diets. Black spots on the larval bodies indicate changes occurring during fungal infection.
Fig 2.
The bacteria identified in the microbial cultures from the cuticle surface of G. mellonella larvae maintained on a semi-artificial (A) and natural (B) diets. Gram staining was used to identify bacteria species.
Fig 3.
Mass spectrum of the trimethylsilyl (TMS) ester of hexadecenoic acid (A) and hexadecanoic acid (B).
Fig 4.
The total ion current (TIC) of fatty acids (TMS esters) of the dichloromethane extract from Galleria mellonella larvae maintained on a semi-artificial (A) and natural diet (B). IS- internal standard- 19-methylarachidic acid; fatty acids and molecular ions: 1- butanoic acid (C4:0, m/z = 160), 2- pentanoic acid (C5:0, m/z = 174), 3- hexanoic acid (C6:0, m/z = 188), 4- heptanoic acid (C7:0, m/z = 202), 5- octanoic acid (C8:0, m/z = 216), 6- nonanoic acid (C9:0, m/z = 230), 7- decanoic acid (C10:0, m/z = 244), 8- dodecanoic acid (C12:0, m/z = 272), 9- tetradecenoic acid (C14:1, m/z = 298), 10- tetradecanoic acid (C14:0, m/z = 300), 11- pentadecanoic acid (C15:0, m/z = 314), 12- hexadecenoic acid (C16:1, m/z = 326), 13- hexadecanoic acid (C16:0, m/z = 328), 14- heptadecenoic acid (C17:1, m/z = 340), 15- heptadecanoic acid (C17:0, m/z = 342), 16- octadecadienic acid (C18:2, m/z = 352), 17- octadecenoic acid (C18:1, m/z = 354), 18- octadecanoic acid (C18:0, m/z = 356), 19- eicozenoic acid (C20:1, m/z = 382), 20- docozanoic acid (C22:0, m/z = 412), 21- heneicosenoic acid (C21:1, m/z = 396), 22- tetracosanoic acid (C24:0, m/z = 440), 23- hexacosanoic acid (C26:0, m/z = 468).
Table 2.
Quantitative summary of the experiment: Numbers of used differently fed Galleria mellonella larvae and masses of extracts.
Table 3.
List of the free fatty acids (FFAs) extracted from the cuticle of G. mellonella larvae maintained on the semi-artificial and natural diet.
All results were calculated as μg/g insect body.