Fig 1.
Relative amino acid concentrations in sperm and seminal plasma obtained from 20 healthy human donors.
The box plots show the minimum, maximum and mean (black cross) of the data. Relative amounts were calculated for each sample based on the total amount of amino acids in the sample and are given in %. Total concentrations (in μmol) are given in the S2 Table.
Fig 2.
Heat map of the correlation coefficients between levels of amino acids from ejaculated mature human sperm and seminal plasma.
Red colored cells represent positive correlations, and blue colored cells represent negative correlations. Only metabolites that were present in ≥ 75% of the samples were considered for correlation analyses.
Fig 3.
Heat map of the correlation coefficients between spermiogram parameters and the levels of amino acids and biogenic amines from ejaculated mature human sperm (A) and seminal plasma (B).
Red colored cells represent positive correlations, blue colored cells represent negative correlations. Only metabolites that were present in ≥ 75% of the samples were included in the correlation analyses.
Fig 4.
Relative acylcarnitine concentrations in sperm and seminal plasma obtained from 20 healthy human donors.
The box plots show the minimum, maximum and mean (black cross) values of the data. The relative amounts were calculated for each sample based on the total amount of acylcarnitines in the sample and are given in %. Total concentrations (in μmol) are given in S2 Table.
Fig 5.
Heat map of the correlation coefficients between spermiogram parameters and the levels of acylcarnitines from ejaculated mature human sperm (A) and seminal plasma (B).
Red colored cells represent positive correlations, and blue colored cells represent negative correlations.
Fig 6.
Relative lysophosphatidylcholine (LPC) concentrations in sperm and seminal plasma obtained from 20 healthy human donors.
The box plots show the minimum, maximum and mean (black cross) values of the data. The relative amounts were calculated for each sample based on the total amount of lipids in the sample and are given in %. Total concentrations (in μmol) are given in S2 Table.
Fig 7.
Relative sphingomyelin (SM) concentrations in sperm and seminal plasma obtained from 20 healthy human donors.
The box plots show the minimum, maximum and mean (black cross) values of the data. The relative amounts were calculated for each sample based on the total amount of lipids in the sample and are given in %. Total concentrations (in μmol) are given in S2 Table.
Fig 8.
Relative phosphatidylcholine (PC) concentrations in sperm and seminal plasma obtained from 20 healthy human donors.
The box plots show the minimum, maximum and mean (black cross) values of the data. The relative amounts were calculated for each sample based on the total amount of lipids in the sample and are given in %. Total concentrations (in μmol) are given in S2 Table.
Fig 9.
Relative etherphosphorylcholine (GPCe) concentrations in sperm and seminal plasma obtained from 20 healthy human donors.
The box plots show the minimum, maximum and mean (black cross) values of the data. The relative amounts have been calculated for each sample based on the total amount of lipids in the sample and are given in %. Total concentrations (in μmol) are given in S2 Table.
Fig 10.
Heat map of the correlation coefficients between spermiogram parameters and the levels of lysophosphatidylcholines (LPC) and sphingomyelins (SM) from ejaculated mature human sperm (A) and seminal plasma (B).
Red colored cells represent positive correlations, and blue colored cells represent negative correlations. Only metabolites that were present in ≥ 75% of the samples were considered in the correlation analyses.
Fig 11.
Heat map of the correlation coefficients between spermiogram parameters and the levels of phosphatidylcholines (PC) and ether-bound glycerophosphorylcholines (GPCe) from ejaculated mature human sperm (A) and seminal plasma (B).
Red colored cells represent positive correlations, and blue colored cells represent negative correlations. Only metabolites that were present in ≥ 75% of the samples were included in the correlation analyses.
Fig 12.
Heat map of the correlation coefficients between spermiogram parameters and the levels of phosphatidylcholines (PC) and ether-bound glycerophosphorylcholines (GPCe) with different double bond contents from ejaculated mature human sperm (A) and seminal plasma (B).
Red colored cells represent positive correlations, and blue colored cells represent negative correlations.