Fig 1.
3MI degradation efficiency of the different strains from chicken manure.
Table 1.
Biochemical and physiological characteristics of NTA1-2A and TAT1-6A strains using the GEN III MicroPlate identification tool.
Table 2.
Chemical sensitivity of strains NTA1-2A and TAT1-6A strains using GEN III MicroPlate identification tool.
Fig 2.
Neighbor joining tree of strains Acinetobacter towneri NTA1-2A and Acinetobacter guillouiae TAT1-6A.
Fig 3.
Proliferation of the strains under different temperature; (A) Acinetobacter toweneri NTA1-2A, (B) Acinetobacter guillouiae TAT1-6A. Symbols: solid circle, 25°C; open circle, 28°C; solid triangle, 31°C; open triangle, 34°C; solid square, 37°C at pH 6 and 1 mM of 3MI concentration.
Fig 4.
Proliferation of the strains under different pH; (A) Acinetobacter toweneri NTA1-2A, (B) Acinetobacter guillouiae TAT1-6A. Symbols: solid circle, pH 5; open circle, pH 6; solid triangle, pH 7; open triangle, pH 8; solid square, pH 9 at 31°C and 1mM of 3MI concentration.
Fig 5.
Effect of T (°C) on 3MI degradation efficiency by the strains at pH 6, and 1mM 3MI concentration.
Symbols: open circle, NTA1-2A strain; solid circle, TAT1-6A strain.
Fig 6.
Effect of pH on 3MI degradation efficiency by the strains at T31, and 1 mM (131.17 mg/L) 3MI concentration.
Symbols: open circle, NTA1-2A strain; solid circle, TAT1-6A strain.
Table 3.
Effect of initial concentration of 3MI on degradation ability of NTA1-2A and TAT1-6A strains.
Fig 7.
3MI removal capability of NTA1-2A strain at different initial concentrations (mg/L).
Symbols: solid circle, 65.58; open circle, 131.17; solid triangle, 196.75; open triangle, 262.34; solid square, 327.92; Error bars, mean ±SE of three riplicates; at T = 31°C and pH 6.
Fig 8.
3MI removal capability of TAT1-6A strain at different initial concentrations (mg/L).
Symbols: solid circle, 65.58; open circle, 131.17; solid triangle, 196.75; open triangle, 262.34; solid square, 327.92; Error bars, mean ±SE of three riplicates; at T = 31°C and pH 6.
Fig 9.
3MI degradation test by extract obtained from medium cultures of NTA1-2A and TAT1-6A.
Error bars: mean ± SE of three replicates.
Table 4.
3MI removal efficiency of NTA1-2A and TAT1-6A from chicken manure.
Values are mean ± SE of triplicate assays.