Table 1.
Coded and real levels of aeration and agitation and the response variables of the R. solanacearum RS inoculum incubated for 24 h at 32°C in YM culture medium, using sucrose as the carbon source.
Fig 1.
Growth curves of the R. solanacearum RS strains, P(3HB) accumulation and sugar and nitrogen residuals.
(A) shaker and bioreactor, cellular concentration expressed as OD600nm; (B) bioreactor, dry cellular weight (DCW) and P(3HB) accumulation (g.L-1); (C) bioreactor, sugar and nitrogen residuals during 48 h (g.L-1).
Table 2.
Analysis of variance (ANOVA) for the quadratic polynomial model fitted for the maximum P(3HB) yield.
Analysis of variance (ANOVA) of aeration and agitation of the response variables of the R. solanacearum RS inoculum incubated for 24 h at 32°C in YM culture medium using sucrose as the carbon source.
Table 3.
Polydispersity index and average Mm for the recovered P(3HB).
Gel Permeation Chromatography (GPC) of the aeration and agitation treatment for the R. solanacearum RS inoculum incubated for 24 h at 32°C in YM culture medium using sucrose as the carbon source.
Fig 2.
(A) Response and contour surfaces as a function of P(3HB) accumulation for the variables aeration and agitation.