Table 1.
Number of positive minor salivary glands (MSG) samples according to the presence of at least one focus scored as possible germinal center (GC)-like structure by the operators (Op) on haematoxylin and eosin (H&E) or by different histological techniques.
Fig 1.
Representative microphotographs of 2 pSS MSGs stained with haematoxylin and eosin (A, E), CD3/CD20 (B, F), CD21 (C, G) and B-cell lymphoma (Bcl)-6 (D, H). In panel A, a light zone surrounded by a dark zone is detected (square) which allows to identify a possible GC-like structure. Panels B and C show the presence of B/T cell segregation and a follicular dendritic cell (fDC) network. However, Bcl-6 is absent (D). In panel E, there are no hallmarks of GC-like structures, however there is B/T cell segregation (F), a fDC network (G) and Bcl-6 positivity (H).
Fig 2.
Distribution of focus score values in all MSG samples and according to the presence or absence of germinal center-like structures.
Fig 3.
Forest plot displaying the Cohen’s kappa values and respective 95% confidence intervals among operators and histological techniques.
Vertical lines identify subgroups and asterisks display statistically significant differences within the subgroups (p<0.05). (Op: operator; H&E: haematoxilin and eosin; segr: segregation).
Table 2.
p values resulting from the Mc Nemar test to identify the distribution of disagreement among operators (Op) and histological techniques.
Significant p values <0.05 identify asymmetric disagreement. H&E, haematoxylin and eosin.