Fig 1.
Crossing and selection scheme for new B. napus types produced from a single hybrid between B. rapa and O. violaceus.
xO: uncertain number of chromosomes from O. violaceus.
Fig 2.
Upper panels: young O. violaceus (P1) and B. rapa (P2) plants; A1–F1: Lines 20, 17, 10, 2, 25, and 8. Lower panel: (A–F): flowering plants of all six lines. The arrow points to a bent main stem.
Fig 3.
Different pollen grain shapes in the progeny lines.
(A) Stainable round and unstainable shrunken pollen grains from Line 5. (B, C) Stainable elliptic pollen grains from Line 23 and O. violaceus, respectively.
Fig 4.
Chromosome complements of different lines, as revealed by BAC-FISH analysis.
Red signals are from the C-genome-specific probe; DAPI and merged images are shown for each cell. (A–D) 2n = 38, 38, 39, and 38 from Lines 23, 28, 12, and 4, respectively, which include 18, 16, 19, and 15 labeled chromosomes (A1–D1). Bar: 10 μm.
Table 1.
Quadrivalents formation in meiotic cells and pollen viability in various lines.
Fig 5.
BAC-FISH analysis of meiotic cells from line 21.
A–D: Inverted images of DAPI staining. A1–D1: Merged images. Red signals are from the C-genome-specific probe. Arrows show quadrivalents or multivalents. The two arrowheads in C1 show two pairs of homologous chromosomes between A and C genome chromosomes.
Fig 6.
SRAP profiles of 39 lines as well as B.rapa and O. violaceus generated from one of the primer pairs (e1m6).
Arrows of a, e indicated the specific bands for O. violaceus, b indicated the specific band for B. rapa, c,d indicated that the new bands not detected in the two parents.