Fig 1.
Frequency distributions of intra- and interspecific pairwise K2P distances calculated using the selected mini-barcode region (Fragment 230_b).
The barcode gap (insert) lies between the genetic distances of 0.01 and 0.02.
Fig 2.
Amplification of four different cytochrome oxidase (COI) primer pairs tested in this study.
Lanes 2, 3, 8, 9, 14, 15, 21, 22, 27 and 28 are PCR products recovered from DNA extracted from adult Panulirus homarus. Lane 4, 5, 10, 11, 16, 17, 23, 24, 30 and 31 are PCR products recovered from DNA extracted from phyllosoma samples. Lane 6, 12, 18, 25 and 31 are PCR negative controls. Lane 1, 19, 20 and 32 are 100 bp molecular weight marker (Solis Biodyne). Lane 7, 13 and 26 had no samples loaded.