Table 1.
Characteristics of selected pathogen-specific serology antigens.
Table 2.
Characteristics of reference serum panels.
Fig 1.
Comparison of quantitative antibody measurements (MFI) with reference serostatus in pathogen-specific Monoplex Serology for HBV (Ia, Ib), HCV (II), HTLV-1 (III) and T. gondii (IV).
Ia, Ib, II-IV indicate corresponding reference panels. red lines: optimized cut-offs for single antigen performance; cut-offs were determined by optimizing specificity and sensitivity analogous to Receiver Operator Characteristics analysis. No cut-offs were determined for antigens that showed a reduced capacity to distinguish between reference assay positives and negatives. MFI: Median Fluorescence Intensities.
Table 3.
Single antigen performance in Monoplex Serology in corresponding reference panels.
Table 4.
Overall pathogen-specific statistical performance in Monoplex Serology in corresponding reference panels.
Fig 2.
Statistical performance of pathogen-specific assays (overall seropositivity to HBV, HCV (AND/OR), HTLV-1, T. gondii) in monoplex (blue) and multiplex (orange) format.
To enhance visualization, Cohen’s kappa statistics are illustrated as percentages. Sensitivity, specificity and Cohen’s kappa statistics are illustrated by boxes while the corresponding 95% CI is indicated by horizontal lines. Performance of Monoplex and Multiplex Serology was directly compared on the corresponding reference panel using ICCs. The comparison yielded moderate to excellent reliability between monoplex and multiplex format: ICCHBV RPIa: 0.94 (95% CI 0.92–0.95), ICCHBV RPIb: 0.97 (95% CI 0.96–0.98), ICCHCV: 0.97 (95% CI 0.96–0.98), ICCHTLV-1: 0.94 (95% CI 0.92–0.95), ICCT.gondii: 0.69 (95% CI 0.61–0.76). kappa: Cohen’s kappa. ICC: Intraclass correlation coefficient. CI: confidence interval. RP: reference panel.