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Fig 1.

Manglietia pubipedunculata.

A, flower branch; B, flower bract; C, outer tepal; D, mid tepal; E-F, inner tepal; G, gynoecium with stamens; H, gynoecium; I, longitudinal section of gynoecium; J, longitudinal section of carpel; K, stamens; L, fruit aggregate.

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Fig 2.

Images of living plants of Manglietia pubipedunculata and M. kwangtungensis.

A-D, G, I, M. pubipedunculata; E, F, H, M. kwangtungensis. A, habit; B-C, flower; D-E, flower bud; F-G, gynoecium; H-I, fruit aggregate.

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Fig 3.

Characteristics of leaf epidermis of Manglietia pubipedunculata and M. kwangtungensis.

A-F, M. pubipedunculata; G-L, M. kwangtungensis. A, G, outline of upper epidermis; B, C, H, I, pubescence of lower epidermis; D, J, pubescence base of lower epidermis; E, F, K, L, stomata apparatus of lower epidermis. Scale bar: A, E, K = 50 μm; B, H = 200 μm; C, G, I = 100 μm; D, F = 10 μm; J = 20 μm; L = 5 μm.

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Fig 4.

Characteristics of leaf structure of Manglietia pubipedunculata and M. kwangtungensis.

A-C, M. pubipedunculata; D-F, M. kwangtungensis. Scale bar: A, D = 200 μm; B, C, E, F = 100 μm.

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Fig 5.

Sclerotesta morphology of Manglietia pubipedunculata and M. kwangtungensis under stereomicroscope.

A, B, M. pubipedunculata; C, D, M. kwangtungensis; A, C, dorsal surface of seeds; B, D, ventral surface of seeds. Scale bar: 1 mm.

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Fig 6.

Epidermal cell morphology of sclerotesta of Manglietia pubipedunculata and M. kwangtungensis under scanning electron microscope.

A, B, M. pubipedunculata; C, D, M. kwangtungensis; A, C, cell pattern and shape of sclerotesta surface; B, D, secondary cell wall sculpture of sclerotesta surface. Scale bar: A, C = 50 μm; B = 5 μm; D = 2 μm.

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Table 1.

Macromorphological comparison between Manglietia pubipedunculata and M. kwangtungensis.

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Fig 7.

The ML consensus tree of Manglietia based on three molecular markers (PHYA, LEAFY, and trnH-psbA).

Note: The topolopy of the MP tree is congruent with the ML tree. Numbers above and below the branches are ML maximum parsimony bootstrap values (BS) and MP maximum parsimony bootstrap values (BS), respectively. [] shows the tree of ML and MP conflicts in the node.

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