Table 1.
Characteristics of non-azoospermic men and specimens screened by FISH aneuploidy.
Fig 1.
Sperm aneuploidy assessed by FISH in specimens of different origins with related pregnancy outcome.
The two-sample t-test and the Mann-Whitney U test were used to compare the autosomal and gonosomal aneuploidy rates assessed by FISH in at least 1000 spermatozoa of ejaculated (n = 87), testicular (n = 4), and epididymal (n = 2) specimens in comparison to the donor control group (n = 2) (Upper graph). Friedman’s Chi-square test was then used to compare the clinical pregnancies and their evolution into miscarriage or term. These outcomes are represented according to the source of spermatozoa used for ICSI (Lower graph). No significant differences were found in both statistical comparisons.
Table 2.
Characteristics of couples and semen specimens screened by NGS aneuploidy.
Fig 2.
Sperm aneuploidy assessed by NGS in specimens of different origins with related pregnancy outcome.
The two-sample t-test and Mann-Whitney U test were used to compare the autosomal and gonosomal aneuploidy rates assessed by NGS in at least 1000 spermatozoa and evidenced that the ejaculated (n = 16) specimen had a higher incidence of aneuploidy than the epididymal (n = 2), testicular (n = 4), and donor control specimen (n = 2) (P<0.0001) (Upper graph). In the lower graph, the clinical pregnancies and their evolution into miscarriage or term, assessed by Friedman’s Chi-square test, are represented according to the source of spermatozoa used for ICSI. No statistical difference was found in this analysis.
Fig 3.
Aneuploidy comparison per chromosome by FISH and NGS.
Aneuploidy assessment by FISH, when compared to NGS, appears minute. This dramatic difference between the two assays demonstrates the sensitivity of the NGS assessment.
Table 3.
Characteristics of sperm specimens from different origin obtained from the same men and screened by FISH and NGS.
Fig 4.
Sperm aneuploidy assessed by FISH and NGS of ejaculated and testicular specimens from the same individuals with related pregnancy outcome.
The two-sample t-test and Mann-Whitney U test were used to compare the autosomal and gonosomal aneuploidy rates assessed concurrently by FISH and NGS, in the same individuals, on at least 1000 spermatozoa. A higher incidence of aneuploidy was found in the ejaculated (n = 3) in comparison to the testicular (n = 3) and donor control specimens (n = 2) (P = 0.02) (Upper graph). In the lower graph, the Friedman's Chi-square used to compare the clinical pregnancy according to the source of spermatozoa used for ICSI did not evidence any statistical difference.