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Fig 1.

Flowchart of improved clustering-based image segmentation method.

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Fig 2.

Examples of image preprocessing with (a) source image, (b) contrast enhanced image, (c) gridding image, and (d) coordinates of gridding lines.

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Table 1.

Features used in our approach.

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Fig 3.

Deficiency examples of different segmentation results.

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Table 2.

Description of six real microarray data sets.

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Fig 4.

Segmentation results of four methods on microarray images drawn from six data sets.

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Fig 5.

Gene expression for images in Fig 4 with four different methods.

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Table 3.

Special gene expression comparison of four methods on 6 data sets.

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Fig 6.

Scatter plot of two channel intensities for four methods on image drawn from DeRisi dataset.

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Fig 7.

Scatter plot of two channel intensities for four methods on image drawn from SMD dataset.

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Fig 8.

M-A plot of four methods on image drawn from DeRisi dataset.

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Fig 9.

M-A plot of four methods on image drawn from SMD dataset.

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Table 4.

The mean, minimum and maximum background-corrected intensities of four methods for two channels on six datasets.

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Fig 10.

Segmentation results of various shape spots.

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Table 5.

Quantum analysis of the segmentation results showed in Fig 10.

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Fig 11.

Examples of segmentation results on simulation images.

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Table 6.

Quantitative evaluation for four methods on simulation image.

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Table 7.

Computational time comparison of five methods on blocks drawn from six data sets (seconds).

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