Fig 1.
Quantification method of fibrin deposition surrounding stent struts.
(A–B) Representative histologic sections stained with hematoxylin and eosin, and a combined Verhoeff and Masson trichrome stains, showing fibrin deposition surrounding stent struts. Fibrin was identified as an intense, homogenous red stain in a combined Verhoeff and Masson trichrome stains (B-lower panel). (C) Areas of neointimal fibrin deposition were digitally detected as green areas (red arrows) and measured with off-line morphometric software (WinROOF image-processing software, Version 6).
Fig 2.
Representative histologic sections stained with a combined Verhoeff and Masson trichrome stains at 7 and 10 days.
Sections show low power (4x) and high power (20x) magnification following a combined Verhoeff and Masson trichrome staining. These images showed fibrin deposition (red arrows) around stent struts.
Fig 3.
Comparison of areas of fibrin deposition across stent types.
Areas of fibrin deposition were significantly greater for DP-EES when compared to BP-SES for both days. A significant difference in areas of fibrin deposition between BP-SES and BP-EES were not found. **p<0.01 versus BP-SES group.
Fig 4.
Comparison of percentage fibrin to internal elastic lamina areas across stent types.
Similar to the results of Fig 3, significant differences between BP-SES and DP-EES with regard to the ratio of fibrin area to IEL areas for both days were noted. Although the ratio for BP-EES was numerically greater than that of BP-SES, a significant difference was not noted. **p<0.01 versus BP-SES group.
Table 1.
Results of Histomorphometric analysis at 7 and 10 day.
Fig 5.
Representative images of transmission electron microscope at 7 and 10 days.
Stents were also investigated for re-endothelialization by transmission electron microscope (TEM) at the Food and Drug Safety Center–Hatano Research Institute. All struts were selected from hematoxylin and eosin-stained specimens for detailed analysis by TEM. TEM images showed endothelial cells (EC) and tight junctions (white arrows) around stent struts.
Table 2.
Transmission Electron Microscopic Findings at 7 days.
Table 3.
Transmission Electron Microscopic Findings at 10 days.