Table 1.
Cy3-labeled oligonucleotide probes used in this study for FISH counting.
Fig 1.
Physico-chemical and turbidity profiles of Lake Cadagno water column.
Oxygen [mg L-1, blue line], H2S [mg L-1], conductivity [mS cm-1] (left), and turbidity profile [NTU] (right) (12 July 2016). The pink shadow highlight the population of anoxygenic phototrophic sulfur bacteria.
Fig 2.
Percentage of relative abundance of bacterial communities at the phylum level according to mixolimnion, chemocline and monimolimnion water layers in Lake Cadagno (October 2012).
Fig 3.
Percentage of relative abundance of bacterial communities at the family and genus level according to mixolimnion, chemocline and monimolimnion water layers in Lake Cadagno (October 2012).
Fig 4.
PSB and GSB FISH quantification in July and October 2016.
Number of cells (cells mL-1) with standard error bar for (A) PSB C. okenii, L. roseopersicina, “T. syntrophicum”, L. purpurea and (B) GSB C. clathratiforme, C. phaeobacteroides. Cell concentrations were determined at maximal turbidity, corresponding to 12.2 meters depth in July and 14 meters depth in October.
Fig 5.
Flow cytometry detection of phototrophic populations in the oxic-anoxic transition of Lake Cadagno scatter plot SSC versus FSC for chlorophyll-pigmented cells (upper panel) and phycocyanin/phycobillin pigmented cells (lower panel).
Chlorophyll and phycocyanin were used as hallmarks for phototrophic microorganisms and cyanobacteria, respectively. Threshold for pigmentation determination was set to FL3 > 1’100 and FL4 > 1’100 for chlorophyll/bacteriochlorophyll and phycocyanin/phycobillin, respectively. P: C. okenii, G: Chlorobium spp., phycobilin: Cyanobacteria (A) July 2016, (B) July 2017, (C) August 2017.
Fig 6.
FCM determined quantification of C. okenii (P), Chlorobium spp. (G), and cyanobacteria (phycobilin) are reported at four depths (± 5%, maximal machine error).