Fig 1.
Percentage of total (A) and progressive motility (B) at different time points of ejaculated (EJ) and epididymal (EP) sperm cultured in fertilization medium in the presence (EP+) or absence of heparin in (EP-). A, B Different letters in the line indicate a difference in time points (0, 3, 6, 12 and 18 hours) within each group, analyzed by Tukey-Kramer test (P≤0.05).
Fig 2.
Percentage intact plasma membrane (A) and live cells with intact acrosome (B) at different time points of ejaculated (EJ) and epididymal (EP) sperm cultured in fertilization medium in the presence (EP+) or absence of heparin in (EP-). A, B Different letters in the line indicate a difference in time points (0, 3, 6, 12 and 18 hours)within each group, analyzed by Tukey-Kramer test (P≤0.05).
Fig 3.
Percentage of cells with stable plasma membrane (A) and potential mitochondrial (B) at different time points of ejaculated (EJ) and epididymal (EP) sperm cultured in fertilization medium in the presence (EP+) or absence of heparin in (EP-). A, B Different letters in the line indicate a difference in time points (0, 3, 6, 12 and 18 hours) within each group, analyzed by Tukey-Kramer test (P≤0.05).
Table 1.
Fertilization rate of bovine oocytes co-incubated for 3, 6, 12 or 18 hours with ejaculated (EJ) and epididymal (EP) sperm in the absence (EP-) and presence of heparin (EP+).
Table 2.
Cleavage on D2 and blastocyst rates on days 6, 7 and 8 of culture using ejaculated sperm selected in Percoll (EJ-P), and epididymal sperm (EP) selected in Percoll (EP-P), PureSperm (EP-PS) or washed in sp-TALP (EP-spTALP).
Fertilization medium of all treatments was supplemented with 10 μg/mL of heparin.
Table 3.
Embryo developmental stages on days 6, 7 and 8 of culture using ejaculated sperm selected in Percoll (EJ-P) and epididymal sperm (EP) selected in Percoll (EP-P), PureSperm (EP-PS) or washed in sp-TALP (EP-spTALP).
Fertilization medium of all treatments was supplemented with 10 μg/mL of heparin.
Fig 4.
Percentage of male and female D8 embryos produced in vitro using ejaculate spermatozoa submitted to the Percoll selection (EJ-P) and epididymal sperm submitted to the Percoll (EP-P), PureSperm (EP-PS) and the wash with spTALP medium (EP-spTALP).
a, b indicate difference between the proportion of male: female embryos within each group, analyzed by chi-squared test (P≤0.05).
Table 4.
Cleavage on D2 and blastocyst rates on days 6 and 7 using ejaculated sperm (EJ-P) or epididymal sperm (EP) co-incubated with oocytes for 6, 12 and 18 h in fertilization medium supplemented with 10 μg/mL of heparin.
Table 5.
Blastocysts developmental stages on days 6 and 7 of culture originated from oocytes co-incubated with ejaculated sperm (EJ 18h) per 18 hours and with epididymal sperm for 6, 12 and 18 h (EP 6h, EP 12h, EP 18h).
Fertilization medium of all treatments was supplemented with 10 μg/mL of heparin.
Table 6.
Total cell number (mean ±SD) and percentage of apoptotic cells (mean ±SD) of day 7 embryos produced from oocytes co-incubated with ejaculated sperm for 18 hours (EJ 18h), and epididymal sperm for 6 (EP 6h), 12 (EP 12h) and 18 h (EP 18h).