Fig 1.
Microfluidic device schematic.
(A) The design of the microfluidic device with the central vascular chamber (yellow) and adjacent stromal chambers (brown). Scavenger channels (blue) are placed next to the stromal chambers and media lines (red) feed the vascular chamber. (B) Experimental setup for the microfluidic devices. A difference in hydrostatic pressure head between the inlet and outlet of each microfluidic line creates a drop in pressure between the two sides of the vascular chamber to induce convective interstitial flow throughout the device.
Table 1.
List of model parameters.
Table 2.
List of experimental conditions.
Fig 2.
Microfluidic device characterization.
(A) Surface map of the steady pressure (Pa) distributions inside the microfluidic device. (B) Surface map of the fluid velocity (μm s-1) and streamlines. (C) FITC-dextran was introduced through the media lines to demonstrate the direction of flow through the device (bottom to top and central chamber to outside chambers). The image was taken 30 minutes after introducing the dye. Scale bar = 200 μm.
Fig 3.
Finite element simulations and measurements of steady state oxygen tension.
(A-C, top row) Surface maps of theoretical oxygen tension in different conditions are shown. (A) Physioxia condition (P) with no oxygen scavenger, (B) constant chronic hypoxia condition (CH) with a constant flow of 0.35 M sodium sulfite flowing at 120 μl min-1, and (C) intermittent hypoxia (IH) condition of alternating 1 M sodium sulfite flowing at 120 μl min-1 for an hour (IHon, left panel) and no flow of oxygen scavenger for an hour (IHoff, right panel). (A-C, bottom) Experimental oxygen maps were constructed using a PhLIM technique for the P (A, bottom), CH (B, bottom), and IH (C, bottom) conditions. The IHon measurements were taken an hour after flowing sodium sulfite, and the IHoff measurements were taken an hour after stopping flow. (D) Temporal variations of the oxygen tension at a point in the left stromal chamber from the COMSOL model in (C, top). The asterisks (*) and circle (o) on the oxygen maps corresponds to the low and high points of the graph, respectively. (E) Comparing the oxygen profiles of all the varying conditions along the central line of the three chambers (arrow in A-C). The conditions are P (black), CH (red), IH (green). The IH case is represented by a green area to illustrate the range of oxygen profiles between the two extreme states of the condition. The solid lines are values from the COMSOL model, and the points are averages from the PhLIM measurements for the three conditions (n = 3 for each conditions).
Fig 4.
(A) Reduced mass flow rate condition (rCH) with 0.07 x 10−6 mol s-1 of sodium sulfite. (B) Increased wall distance (wCH) between the scavenger channel and stromal chamber to 60 μm. (C) Two active scavenger lines (2CH). (D) Comparing the oxygen profiles of all the varying conditions along the central line of the three chambers (arrow in A-C) with the experimental conditions from Fig 3. The conditions are P (black), CH (red), IH (green), rCH (yellow), wCH (purple), and 2CH (blue).
Fig 5.
Biased angiogenesis due to hypoxia.
(A) Representative images from the P (top), CH (middle), and IH (bottom) conditions. In each category, images of the vascular network before (left) and after (right) the condition was applied is shown. (B) The total vessel area for each category in the left and right stromal chambers were measured and compared. For both hypoxic conditions, the vessel area in the left stromal chamber (closer to the scavenger channel) was significantly higher than the right chamber. (C) Biased angiogenesis was calculated for the left and right stromal chambers in each condition and compared to each other. Both hypoxic conditions had significantly more bias in the left stromal chamber. P, n = 8; CH, n = 11; IH, n = 17. Scale bar = 200μm * p < .05, ** p < .005, *** p < .0005, **** p < .0001.