Fig 1.
Diagram of simplified valve margin pathway for fruit dehiscence in Arabidopsis thaliana; valve margin.
R, replum. Sl, separation layer. ll, lignification layer. Valve margin = sl + ll. Modified from data available in [7–8,11] and Fig 2 [34].
Fig 2.
Mature and young heteroarthrocarpic fruits.
(A), Mature Erucaria erucarioides fruit in lateral view before dehiscence (left), and medial view after dehiscence (right). (B), Young E. erucarioides fruit representing size sampled for transcriptomics in medial view. C, Cakile lanceolata fruit in lateral view before joint abscission (left), and medial view after joint abscission (right). (D), Young C. lanceolata fruit representing size sampled for transcriptomics in medial view; Modified from Fig 1 [34]. White arrows indicate joint region; blue arrows indicate replum. Scale bars = 5mm.
Table 1.
Statistics for de novo Trinity assembly of Erucaria erucarioides and Cakile lanceolata pairwise reads for all isoforms.
Numbers in parentheses refer to longest isoform only.
Fig 3.
Graph of select Gene Ontology (GO) terms for Erucaria erucarioides (grey) and Cakile lanceolata (white). GO counts based on merged profile of three biological replicates per region. Sample (n) and total (N) raw counts log2 transformed for interspecies comparison. GO terms chosen based on search terms: lignin, abscission, dehiscence, and response to hormone.
Fig 4.
Venn diagrams of three-way and pairwise High Throughput Sequencing (HTS) filtered transcripts for Erucaria erucarioides and Cakile lanceolata transcriptomes.
(A), Three-way Venn diagrams of Erucaria and Cakile orthologous clusters for distal, joint, and proximal regions. (B), Pairwise Venn diagrams of Erucaria and Cakile orthologue-clustered transcripts (Erucaria region vs Cakile region).
Fig 5.
Heatmap of all significant edgeR contig clustered transcripts in the Erucaria erucarioides (n = 15,345) and Cakile lanceolata (n = 74) transcriptomes, expressed as z-scores (FDR-corrected α = 0.01).
Fig 6.
Heatmap of edgeR contig clustered transcripts from Erucaria erucarioides and Cakile lanceolata expressed in log2 (TPM) with TMM normalization.
Representative transcripts are those with the highest bitscore hit against the TAIR database. Bolding indicates shared orthogroup with other transcriptome. FULa,b,c,d are copies of FUL that are present in some species across the Brassicaceae [71]. TPM, Transcripts Per Million; TMM, Trimmed Mean of M-values. Asterisks indicate significant differential expression between proximal and joint region. (FDR-corrected α = 0.01).