Fig 1.
The schematic of three bioreactors.
(a) Uniaxial cyclic stretch bioreactor (b) Equiaxial cyclic stretch bioreactor (c) Shear stress bioreactor.
Fig 2.
Effect of strain rate on transfection efficiency (loading time = 60 min, frequency = 0.5 Hz).
Fig 3.
Effect of various loading time on transfection efficiency (5% strain, frequency = 0.5 Hz).
Fig 4.
Effect of simultaneous use of uniaxial cyclic stretch and chemical reagent (TurboFect transfection reagent) for various loading time (strain rate = 0.5%, frequency = 0.5 Hz).
Fig 5.
Effect of uniaxial cyclic stretch on TC-1 transfection efficiency by flow cytometry (loading time: 15 min, frequency: 0.5 Hz, and strain: 5%).
(a) without reagent (b) with reagent.
Fig 6.
The transfection efficiency of pEGFP-N1 in TC-1 related to 15 min loading time for simultaneous use of mechanical loading of uniaxial cyclic stretch and chemical reagent by fluorescent microscopy.
Fig 7.
Comparison of efficiency of equiaxial cyclic stretch transfection methods with and without reagent (frequency 0.5 Hz, strain rate of 5% and loading time of 15 minutes).
Fig 8.
Flow cytometry analysis for equiaxial cyclic stretch transfection of TC-1 (loading time: 15 min, frequency: 0.5 Hz, and strain: 5%).
(a) without reagent (b) with reagent.
Fig 9.
Effect of simultaneous use of mechanical loading of shear stress and chemical reagent (TurboFect Transfection Reagent) for various frequency (loading time = 15 min).
Fig 10.
Effect of shear stress on TC-1 transfection efficiency by flow cytometry (loading time: 15 min, and frequency: 0.5 Hz).
(a) without reagent (b) with reagent.