Fig 1.
A Day of wound closure. Depicted is day of wound closure of controlwounds, ischemic wounds and ischemic wounds after treatment with 11,12 and 14,15 EET. B Representative in vivo pictures of wound healing taken every second day (data is shown as mean ± SD; n = 10). *p<0.05, **p<0.01, ***p<0.001.
Fig 2.
A Percentage of closed wound area from day 0–16 of ischemic and non-ischemic ears. B Percentage of closed wound area from day 0–16 of ischemic and 11,12 EET treated wounds. C Percentage of closed wound area from day 0–16 of ischemic and 14,15 EET treated wounds. (data is shown as mean ± SD; n = 10).*p<0.05, **p<0.01, ***p<0.001.
Fig 3.
A Percentage of VEGF positive area on day 3, 6, and 9 after wounding ofcontrol, ischemic and 11,12 as well as 14,15 EET treated ischemic wounds. On the right representative pictures of immunohistological staining. B Percentage of Ki67 positive cells on day 3, 6 and 9 after wounding ofcontrol, ischemic and 11,12 as well as 14,15 EET treated ischemic wounds. On the right representative pictures of immunohistological staining (data is shown as mean ± SD; n = 8). *p<0.05, ***p<0.001.
Fig 4.
A Percentage of TNF-α positive area on day 3, 6, and 9 after wounding of control, ischemic and 11,12 as well as 14,15 EET treated ischemic wounds. On the right representative pictures of immunohistological staining. B Percentage of TGF-β positive area on day 3, 6 and 9 after wounding of control, ischemic and 11,12 as well as 14,15 EET treated ischemic wounds. On the right representative pictures of immunohistological staining (data is shown as mean ± SD; n = 8). *p<0.05, ***p<0.001.
Fig 5.
A: Percentage of MMP7 positive area on day 3, 6 and 9 after wounding of control, ischemic and 11,12 as well as 14,15 EET treated ischemic wounds. On the right representative pictures of immunohistological staining. B: Percentage of MMP9 positive area on day 3, 6 and 9 after wounding of control, ischemic and 11,12 as well as 14,15 EET treated ischemic wounds. On the right representative pictures of immunohistological staining (data is shown as mean ± SD; n = 8). *p<0.05, **p<0.01, ***p<0.001.
Fig 6.
A: Percentage of TIMP1 positive area on day 3, 6 and 9 after wounding of control, ischemic and 11,12 as well as 14,15 EET treated ischemic wounds. On the right representative pictures of immunohistological staining. B: Percentage of SDF-1α positive area on day 3, 6 and 9 after wounding of control, ischemic and 11,12 as well as 14,15 EET treated ischemic wounds. On the right representative pictures of immunohistological staining (data is shown as mean ± SD; n = 8). *p<0.05, **p<0.01, ***p<0.001.