Fig 1.
Characterization of EHEC O157:H7 proteome and correlation with in silico data.
(A) Correlation of the proteomic results with in silico data of EHEC O157:H7 genome. (B) Dynamic range based on the emPAI value of the proteins identified by LC-MS analysis; pink, most abundant proteins; green, less abundant proteins and red, proteins related to EHEC O157:H7 and that are present in the LEE pathogenicity island.
Table 1.
List of the most abundant proteins of EHEC O157:H7 proteome.
Fig 2.
Functional analysis of the EHEC O157:H7 proteome.
(A) Proteins classified by COG functional categories (B) Categorization of the proteins identified into biological processes. [C] Energy production and conversion; [E] Amino acid transport and metabolism; [D] Cell cycle control, cell division, chromosome partitioning; [G] Carbohydrate transport and metabolism; [I] Lipid transport and metabolism; [J] Translation, ribosomal structure and biogenesis; [K] Transcription; [L] Replication, recombination and repair; [M] Cell wall/membrane/envelope biogenesis; [N] Cell motility; [O] Posttranslational modification, protein turnover, chaperones; [P] Inorganic ion transport and metabolism; [R] General function prediction only; [S] Function unknown; [T] Signal transduction mechanisms; [U] Intracellular trafficking, secretion, and vesicular transport; [V] Defense mechanisms; [X] Mobilome: prophages, transposons. (C) KEGG pathway enrichment analysis, the colors are based on the protein abundance; blue, most abundant and green, less abundant.
Table 2.
List of the most abundant proteins detected in E. coli K-12 and EHEC 86–24.
Fig 3.
Overview of the glycolysis / gluconeogenesis pathway of EHEC O157:H7.
Enzymes of the Glycolysis / Gluconeogenesis metabolism that were identified at the proteome level. Blue, proteins detected in our proteomic analysis; Green, proteins not identified in our study and Red, proteins detected as most abundant.