Fig 1.
Phylogenetic reconstruction of Borrelia species based on 791 aligned protein homologs built with the PEPR pipeline and FastTree2 with 100 jackknifed resampling replicates.
All node support values are 100 except where indicated.
Table 1.
Borrelia species and strains included in study.
Fig 2.
Boxplot of inter- and intra-specific POCP values.
Inter-specific and intra-specific comparisons included Lyme borreliosis (LB) and relapsing-fever species (RF), reptile-associated species (REP) including the echnida-associated species ‘Candidatus B. tachyglossi’. The inter-genera comparison included the members of the genera Borrelia, Brachyspira, Leptospira, Spirochaeta, and Treponema.
Fig 3.
Percentage of conserved proteins (POCP) matrix generated by the method described in [18].
POCP values of species belonging to the LB group, RF group of spirochetes, the reptile-associated species B. turcica and echnida-associated species B. tachyglossi are above the genus threshold of 50%, indicating that all belong into one bacterial genus, Borrelia.
Fig 4.
Partial amino acid alignment of (A) a putative lipoprotein (GI: 1195064) and (B) a hypothetical protein (GI: 1194969) showing a CSI in which the form of the indel in ‘Candidatus Borrelia tachyglossi’ and B. turcica matches that in LB species.
Table 2.
Designation of LB and RF-differentiating CSIs in ‘Candidatus Borrelia tachyglossi’ and B. turcica genomes.