Table 1.
List of bacterial strains used in this study.
Fig 1.
Maximum parsimony tree based on nucleotide sequences of the 16S rRNA gene of Pseudomonas species and pathovars obtained from heuristic parsimony search and bootstrap analysis.
Bootstrap values are shown at the nodes based on 10,000 replications. Gaps were treated as missing data. The 16S rRNA gene sequence of Pseudomonas putida obtained from NCBI database was treated as an outgroup. Bar, 0.001 substitutions per nucleotide position. The accession numbers are listed adjacent to the respective bacterial strain. The “*” in the figure represents a pathotype strain.
Fig 2.
Maximum parsimony tree based on nucleotide sequences of housekeeping genes gltA, gap1, gyrB, and rpoD of Pseudomonas species and pathovars obtained from heuristic parsimony search and bootstrap analysis.
Bootstrap values are shown at the nodes based on 10,000 replications. Gaps were treated as missing data. Bar, 0.01 substitutions per nucleotide position. The accession numbers in the figure are in following order for each strain: rpoD, gap1, gltA and gyrB. The “*” in the figure represents a pathotype strain.
Table 2.
Polymerase chain reaction screening of bacterial strains using of plasmid (pCOR1)- and coronafacate ligase (cfl) gene-specific primers.
Table 3.
Polymerase chain reaction screening of bacterial strains using hrpZ Group I-IV specific primers.
Table 4.
List of effector proteins and aviruluence genes screened by polymerase chain reaction for the bacterial strains of Pseudomonas coronafaciens and Pseudomonas syringae pv. syringae.
Table 5.
DNA relatedness among Pseudomonas coronafaciens and P. syringae pv. syringae.
Table 6.
Phenotypic characteristics that distinguish P. coronafaciens from P. syringae and P. syringae pathovars.
Data for reference taxa for column 3–7 were taken from Schaad et al. (2001).
Table 7.
Pathogenicity test of Pseudomonas coronafaciens, Pseudomonas syringae pv. syringae and yellow bud strains on cereals, grasses and onion.