Fig 1.
A) AAT catalysis. B) Inhibitors of AAT.
Table 1.
Metabolites with at least one set of measurements with p < 0.1.
The ratios of the detected metabolite levels in samples treated with 10 μM hydrazinosuccinic acid versus untreated controls are given. The data is sorted on log2 average ratios and colorized from red (metabolites increased in treated samples) to blue (metabolites decreased in treated samples). Measurements with p-values < 0.05 are highlighted in bold.
Fig 2.
PLS-DA of the pooled data, mean centered and scaled to unit variance; 3 significant components, explained variation in metabolite data R2X = 0.640, explained between class variation R2Y = 0.989, predicted between class variation according to cross-validation Q2 = 0.886 A) Scores plot of the two first components (t2/t1) shows a separation between controls (blue) and treated samples (green). B) Corresponding overview of the two first components (w*c[2]/w*c[1]) showing an overview of the contribution of each metabolite (green) to the model in relation to the two sample classes; controls (DA(1), upper right quadrant) and treated samples (DA(2), lower left quadrant). C) List of all metabolites with p < 0.1 (student’s two-sided unpaired t-test) for the pooled data with corresponding Log2-fold change and p-value. D) Biosynthesis of 4-aminobutyric acid.