Table 1.
Water quality analyses of CTL and RAW seawater.
Table 2.
Proximate (mg g sample-1 DW) and lipid class composition (mg g lipid-1) of exogenous diets.
Table 3.
Fatty acid (mg g lipid-1) and amino acid (mg g sample-1 DW) composition of exogenous diets.
Fig 1.
Effect of five different feeding regimes on the a) growth, b) zooxanthellae density, c) total protein, and d) total lipid concentration of Acropora millepora after 90 days. Boxes encompass the 25th and 75th percentile, dots denote single outliers. n = 3. Boxes in the same plot that do not share the same letters are significantly different (P<0.05). ART = Artemia nauplii, ATF = artificial diet, CTL = ultra-filtered (0.04 μm) seawater, RAW = unfiltered seawater, RR = Reef Roids.
Fig 2.
Effect of five different feeding regimes on the a) growth, b) zooxanthellae density, c) total protein and, d) total lipid concentration of Pocillopora acuta after 90 days. Boxes encompass the 25th and 75th percentile, dots denote single outliers. n = 3. Boxes in the same plot that do not share the same letters are significantly different (P<0.05). ART = Artemia nauplii, ATF = artificial diet, CTL = ultra-filtered (0.04 μm) seawater, RAW = unfiltered seawater, RR = Reef Roids.
Fig 3.
Effect of five different feeding regimes on the lipid class composition of a) Acropora millepora and b) Pocillopora acuta after 90 days (mg g lipid-1). WAX = wax ester, TAG = triacylglycerol, FFA = free fatty acids, 1,2DAG = 1,2diacylglycerol, ST = sterol, AMPL = acetone-mobile-polar-lipid, PE = phosphatidylethanolamine, PS-PI = phosphatidylserine-phosphatidylinositol, PC = phosphatidylcholine. Values are presented as means ± SEM. n = 3. Letters denote significant differences between treatments for each lipid class (P <0.05). ART = Artemia nauplii, ATF = artificial diet, CTL = ultra-filtered (0.04 μm) seawater, RAW = unfiltered seawater, RR = Reef Roids.
Fig 4.
Effect of five different feeding regimes on the major fatty acid class composition of a) Acropora millepora and b) Pocillopora acuta after 90 days (mg g lipid-1). SFA = saturated fatty acids, MUFA = monounsaturated fatty acids, PUFA = polyunsaturated fatty acids. Values are presented as means ± SEM. n = 3. Letters denote significant differences between treatments for each fatty acid class (P <0.05). ART = Artemia nauplii, ATF = artificial diet, CTL = ultra-filtered (0.04 μm) seawater, RAW = unfiltered seawater, RR = Reef Roids.
Fig 5.
Effect of five different feeding regimes on the major long-chain polyunsaturated fatty acid composition of a) Acropora millepora and b) Pocillopora acuta after 90 days (mg g lipid-1). ARA = arachidonic acid (20:4n-6), EPA = eicosapentaenoic acid (20:5n-3), DHA = docosahexaenoic acid (22:6n-3). Values are presented as means ± SEM. n = 3. Letters denote significant differences between treatments for each fatty acid (P <0.05). ART = Artemia nauplii, ATF = artificial diet, CTL = ultra-filtered (0.04 μm) seawater, RAW = unfiltered seawater, RR = Reef Roids.