Table 1.
Determination of the content of humidity, water activity, total ash, raw protein, total lipids and fiber of brown, green and red propolis samples.
Table 2.
Determination of the content of total phenolics (mg EAG/g), flavonoids (mg EQ/g) and the antioxidant activity by DPPH (IC50) of extracts of three different samples obtained by ethanolic (EtOH) and supercritical (SFE) extraction.
Table 3.
Determination of the content of chatequin, trans ferulic acid and luteolin of red, green and brown propolis extracts obtained by ethanolic extraction (EtOH) and by SFE (SCO2).
Fig 1.
Chromatograms of green propolis ethanolic extract–(1) chatequin, (3) trans-ferulic acid and (4) luteolin.
Table 4.
Bibliographical review of recent studies on the quantification of the compounds catechin, ferric acid and luteolin in extracts of propolis from different regions.
Table 5.
Determination of MIC (μg.mL-1) of the extracts from different samples of propolis obtained by ethanolic extraction (EtOH) and by supercritical extraction (SCO2).
Fig 2.
Cell cytotoxicity assessment by Alamar Blue.
The data represents the viability of uninfected macrophages treated for 24 h with medium (Ctr) and the with the ethanolic extracts of propolis. The experiments were performed at least three times in quadruplicate for each experimental group. Data are shown as the mean +/- SD and are representative of three experiments.
Fig 3.
Reduction of the viability L. braziliensis promastigotes after treatment with extracts.
Parasites were incubated with media alone or with ethanolic propolis extracts for 5 days. The viable parasites were counted daily with a Neubauer Chamber. The experiments were performed in quadruplicate for each experimental group (*p <0.05 and **p <0.01). Data are shown as mean +/- SD and are representative of two experiments.