Table 1.
Baboon characteristics.
Fig 1.
Study schema for IV busulfan to induce reversible myelosuppression without stem cell rescue in a pre-clinical baboon model.
Fig 2.
Peripheral blood differential counts post intravenous (IV) busulfan treatment is shown along automated white blood cell (WBC) counts.
A. Peripheral blood absolute neutrophil count (ANC), absolute lymphocyte counts (ALC) and WBC count following 6.4mg/kg IV busulfan treatment (Group A). B. Peripheral blood ANC and WBC counts following 8 mg/kg IV busulfan treatment (Group B). C. Peripheral blood ANC, ALC and WBC counts following 9.6 mg/kg IV busulfan treatment (Group C).
Fig 3.
Effects of intravenous (IV) busulfan on peripheral blood (PB) parameters.
A. Platelet counts in the PB are shown over the study period following busulfan treatment in all three groups of baboons. B. Hemoglobin (Hg) levels following busulfan treatment in all three groups of baboons is shown over the 90-day study period.
Table 2.
Morphologic examination of Bone marrow biopsy specimens collected prior to and following busulfan treatment in baboon.
Fig 4.
Bone marrow CD34+ cells kinetics following IV busulfan treatment in baboons.
A. Frequency (%) of CD34+ cells in bone marrow following IV busulfan treatment over the course of the 90-day study period. B. Absolute number of bone marrow CD34+ cells following IV busulfan treatment for each group of baboons. C. Suppression of bone marrow CD34+ cell absolute values for each group of baboons 15-day post busulfan treatment relative to pretreatment levels are shown as percent values of mean ± SE in a bar graph. D. Recovery of absolute number of bone marrow CD34+ cells 90 after days of busulfan treatment also presented in a scatter graph using mean ± SE values of percent recovery relative to pretreatment levels.
Fig 5.
Alteration of the number of colony forming units (CFUs) in the bone marrow (BM) following intravenous (IV) busulfan treatment over the 90-day study period.
A. The kinetics of frequency of CFUs in the BM following busulfan treatment depicted as plating efficiency (%) over the course of the 90-day study period. B. The kinetics of absolute number of BM CFUs following busulfan treatment for each group of baboons. C. Suppression of absolute number of CFUs in the BM using mean ± SE of percent suppression for each group of baboons 15-day post busulfan treatment to pretreatment levels. D. Recovery of CFUs in the BM 90 days after busulfan treatment relative to pretreatment levels are also shown using mean ± SE values of percent recovery relative to their pretreatment values. E. Correlation coefficient of bone marrow CFU recovery and CD34+ cell content recovery (excluding the outlier); F. Correlation coefficient of BM CFU recovery and recovery of absolute neutrophil count (ANC) in peripheral blood (PB) is shown; G. Correlation coefficient of BM CFU recovery and recovery of white blood cell (WBC) in peripheral blood (PB) is shown.