Fig 1.
Temporal evolution of eosinophils after reperfused ST-segment elevation myocardial infarction and in relation to edema, microvascular obstruction (MVO), infarct size, and major adverse cardiac events (MACE).
Temporal evolution of eosinophils (x1000 cells/ml) (A) in the whole study group and in relation to (B) edema, (C) MVO, (D) infarct size and (E) MACE (cardiac death, re-infarction, or readmission for heart failure) during follow-up. Data are expressed as median with the interquartile range (n = 620) and were analysed by Mann-Whitney U-test. (F) Eosinophil minimum count and outcome Kaplan-Meier curves for survival free of MACE (cardiac death, re-infarction, and readmission for heart failure) during follow-up depending on low eosinophil count. Abbreviations: LV: left ventricular.
Table 1.
Baseline characteristics, eosinophil counts, and cardiac magnetic resonance (CMR) characteristics of the whole study group.
Fig 2.
Dynamics of circulating eosinophils in swine subjected to reperfused myocardial infarction (MI).
Whole blood was isolated from the experimental model at basal and at different time points of the ischemia and reperfusion process: after 5-min and 85-min of ischemia (immediately before reperfusion) as well as 5-minutes, 30-minutes, 7-days, and 1-month after reperfusion post-MI. Samples were incubated with FITC-CD45 and PE-CD16 and afterwards measured using flow cytometry. (A) Eosinophil counts in whole blood were analysed. Data were expressed as median with the interquartile range (n≥5 independent experiments) and were analysed by Kruskal-Wallis analysis followed by Dunn’s test. *P<0.05 vs. basal. (B) Dynamics of peripheral eosinophils in ST-elevation MI-patients (red) and in the swine model (green). The drop in peripheral eosinophils detected in ST-elevation MI-patients 12h after coronary reperfusion might be preceded by their progressive increase during ischemia and soon after reperfusion, as observed in the experimental model.
Fig 3.
Eosinophil mobilization into the infarcted myocardium in a controlled swine model of reperfused myocardial infarction (MI).
(A) Representative images from infarcted tissue isolated from control and three MI groups (90-min of ischemia followed by 3-days, 7-days, and 1-month reperfusion) stained with hematoxylin-eosin (HE) (first panel). The presence of eosinophils was revealed by staining myocardial samples with Luna’s technique, specific for eosinophil granules (second panel) and with the eosinophil-specific protein eosinophil major basic protein (EMBP) (third panel). (B) Quantification of eosinophil cells in the myocardial tissue. Images from the infarcted area isolated from the four independent groups were quantified with Image-Pro Plus analysis software. Scoring was performed by a blinded observer unaware of the experimental group. (C) The expression of eosinophil peroxidase (EPO) in the infarcted myocardium at different times of the ischemia and reperfusion process. Data (mean±SD, n≥4) were analysed by one-way ANOVA analysis followed by Bonferroni test. *P<0.05, **P<0.01 vs. control.
Fig 4.
Infiltration of eosinophils into the infarcted areas with microvascular obstruction (MVO).
(A) Quantification of infiltrated eosinophils according to the extension of MVO. Animals from the 3-days and 7-days reperfusion groups were categorized according to MVO (extensive: MVO> 5% of area at risk, median value) and the number of eosinophils was morphometrically quantified. (B) Quantification of infiltrated eosinophils in samples obtained from the same heart but comparing infarcted areas with macroscopic MVO and without MVO from the same heart.
Fig 5.
Evolution of crucial cytokines implicated in eosinophil maturation, activation, and recruitment.
The serum levels of interleukin (IL)-5 (A), eosinophil cationic protein (ECP) (B), and eotaxin-1 (C) were determined in samples isolated from control subjects (n = 10) and from ST-elevation myocardial infarction (STEMI)-patients (n = 14) upon arrival and after primary coronary intervention (PCI) (24h, 96h, and 1-month). The expression in the infarcted myocardium of IL-5 (D), and eotaxin-1 (F) were obtained at different times of the ischemia and reperfusion process. The correlation of IL-5 and eotoxin-1 with the expression of eosinophil peroxidase (EPO) (E,G, respectively) was assessed using Pearson correlation coefficient. Continuous normally distributed data are expressed as mean±SD and were analysed by one-way ANOVA analysis followed by Bonferroni test. *P<0.05, ***P<0.001 vs. control; +P<0.05 vs. 24h post-PCI. Non-parametric data were expressed as the median with the interquartile range and were analysed by Kruskal-Wallis followed by Dunn’s test. &P<0.05 vs. control. Abbreviations: EMBP: eosinophil major basic protein.
Fig 6.
The presence of eosinophils in autopsies from patients with chronic myocardial infarction (MI).
Representative images from infarcted tissue isolated from control and chronic MI patients stained with hematoxylin-eosin (HE) (left panel), and picrosirius red (middle-left panel). The presence of eosinophils was revealed by staining myocardial samples with the eosinophil-specific protein eosinophil major basic protein (EMBP) (middle-right panel) and with Luna’s technique, specific for eosinophil granules (right panel).