Table 1.
Primers and probe sets designed to target invA and the selected gene fragments of STM4200 and SEN1392.
Table 2.
Detection of the invA, STM4200 and SEN1392 genes for 225 Salmonella strains by the developed multiplex qPCR methods, where (+) indicates detection of the gene and (-) indicates the gene is not detected.
Table 3.
Testing to determine the absence of the invA, STM4200 and SEN1392 genes for 34 non-Salmonella reference strains by the developed multiplex qPCR method, where (+) indicates detection of the gene and (-) indicates the gene is not detected.
Fig 1.
Standard curves for targets invA, SEN1392, and STM4200.
invA: green line and symbols; SEN1392: red line and symbols; STM4200: blue line and symbols.
Fig 2.
Cell standard curves for target invA from S. Typhimurium STM4200 for the matrices BPW, Chicken, and curcuma spice.
BPW: Blue line and symbols; Chicken: red line and symbols; curcuma spice: green line and symbols.
Table 4.
Level of detection for various artificially contaminated matrices for the qPCR method, the ISO method and the MSRV method.