Fig 1.
Length distribution of unigenes and transcripts in base pairs.
Table 1.
Summary of transcriptome data analysis of Hirudo nipponia.
Fig 2.
Characteristics of gene annotation according to the Nr database.
(A) E-value distribution of Blastx hits for unigenes with a cut-off E-value of 1.0e-5. (B) Similarity distribution of Blastx hits for unigene. (C) Species classification is shown as a percentage of the total homologous sequences with an E-value of at least 1e-5.
Fig 3.
Gene Ontology (GO) categories of genes from H. nipponia salivary glands.
GO functional annotations are summarized in three main categories: biological process, cellular component and molecular function. Each category represents a GO term assigned by Blast2GO analysis.
Fig 4.
KOG annotation of putative proteins.
Table 2.
Top 20 predicted KEGG pathways in the Hirudo nipponia sialotranscriptome.
Table 3.
Anticoagulants included in the locally compiled data set used for the BLASTx comparisons.
Fig 5.
Alignment of inferred amino acid sequences for Hirudo nipponia transcripts corresponding to well-characterized leech salivary bioactive peptides.
Similar residues are shaded, with the highlighted homology level ranging from dark black (100% identity), black (75–100% identity), grey (50–75% identity), to light grey (33–50% identity). Red boxes correspond to predicted secretory signal peptides. Green boxes outline conserved cysteines. Hnip, Hirudo nipponia; Hmed, Hirudo medicinalis; Pvir, Poecilobdella viridis; Hman, Hirudinaria manillensis; Hoff, Haementeria officinalis; Hghil, Haementeria ghilianii. *The sequences of H. nipponia described in this study.