Table 1.
Sequenced and aligned reads of the analyzed honeys.
Fig 1.
Effect of minimum sequence identity cut-off values on the number of mapped sequenced reads (considering the genus and species levels).
A) Orange tree blossom honey, genus level; B) Orange tree blossom honey, species level; C) Eucalyptus tree blossom honey, genus level; D) Eucalyptus tree blossom honey, species level. Colors are as follow: blue, arthropods; yellow, plants; gray, fungi; orange, bacteria; green, viruses. Data are not reported for viruses at the genus level since this level is missing for some of them, leading to unreliable statistics. Plots are reported for a sequence identity ranging from 100 to 70% (from the left to the right) as lower values did not change the number of assigned sequences (as evidenced from the plateau reached at all levels).
Table 2.
Lowest percentage of sequence identity below which the comparison against the NCBI nt database did not report any decrease in the number of annotated reads and identified taxa.
Fig 2.
Proportion of reads assigned to the five organism groups in the orange tree and eucalyptus tree blossom honeys using different sequence identity.
Orange tree blossom honey: sequence identity ≥ 75% (A) and ≥ 97% (B); eucalyptus tree blossom: sequence identity ≥ 75% (C) and ≥ 97% (D). Percentages are computed considering only reads annotated at the taxonomic rank of species (see Table 3).
Table 3.
Number of putative MOTUs at the species and genera levels characterizing the two honey samples.
Data are presented for each dataset, considering three different thresholds of sequence identity (<75%, that means without any threshold; ≥ 75%; ≥ 97%).
Table 4.
Predominant species identified in the two analyzed honeys considering the ≥75% sequence identity level based on reads accounting >5% overall reads of the identified organism groups.
Fig 3.
Proportion of reads assigned to distinct bacteria families (derived by the sum of reads of the species belonging to the family) in the two analyzed honeys.
Orange tree blossom honey: sequence identity set at ≥75% (A) and ≥97% (C); eucalyptus tree blossom honey: sequence identity set at ≥75% (B) and ≥97% (D). “Others” indicates all other families detected that did not pass the 1% of reads over all bacteria reads.