Table 1.
Overview of the performed technologies.
Fig 1.
Flow diagram of the study design.
Two transfection experiments and exon skipping quantification were performed. AON = antisense oligonucleotide, BOM-SG = biochemical outcome measures group, ddPCR = digital droplet PCR and qPCR = quantitative real-time PCR.
Fig 2.
Bar graphs showing exon skipping levels of DMDΔ48–50 (A-D) and DMDΔ52 (E-H) cells after transfection with AON h51AON2 to skip exon 51 (1st transfection experiment).
Four different protocols were tested. Error bars represent standard deviation.
Fig 3.
Bar graphs showing exon skipping levels of DMDΔ48–50 (A-B) cells after transfection with AON h51AON2 to skip exon 51 (2nd transfection experiment).
Two different protocols were tested. Error bars represent standard deviation.
Fig 4.
Boxplots of log transformed exon skipping levels (y-axis) are shown per technology (x-axis).
The figure is divided into three horizontal panels representing the different sets of samples and four vertical panels illustrating the AON concentrations used for the transfections. Exon skipping levels of the different labs are shown in colours (lab 1 = yellow, lab 2 = red, lab 3 = green, lab 4 = turquoise, lab 5 = blue and lab 6 = pink). Outliers are represented by coloured dots.
Table 2.
P-values obtained for the double generalized linear model.