Table 1.
Pollen fertility investigated by different methods.
Fig 1.
Anther morphology and pollen fertility of D248A (A, D) and D248B (B, C). (a, b) Anther, (c, d) Pollen grain stained by FDA. Magnitude power was set to ×100 for (c-f).
Fig 2.
Cytological characterization of anthers and pollen grains using scanning electron microscopy.
(a, c, e, g) D248B, (b, d, f, h) D248A. (a, b) anther, (c, d) xx, (e, g, f, h) pollen.
Fig 3.
Micrographs of anther development in D248A (A-D, I-L) and D248B (E-H, M-P) using light microscopy. (a, b, e, f) sporogonial stage. (c, g) microspore mother cell stage. (d, h, i, m) tetrads stage. (j, k, n, o) microspore stage. (l, p) mature pollen stage. Abnormality of anther development began at the tetrads stage (d, i) and peaked at the microspore stage (j, k) as evidenced by the deformation and degeneration of both the tapetum and microscope. En endothecium, Ep epidermis; M middle cell layer; Sp sporogenous cell, Ms microspore, Mc meiotic cell, Msc microsporocyte; PG pollen grain, T tapetum, Td tetrads. Bars = 60 μm. The magnification power was 10×20 in b and f, 10×40 in a, c-e, h-p.
Fig 4.
Genetic and physical mapping of the male sterility locus (Ms) in D248A.
Sesame genome sequence version 2.0 (http://www.sesame-bioinfo.org/Sinbase2.0/) was used for physical mapping and gene prediction.
Table 2.
Primers sequences for markers linked to male sterility in D248A.
Table 3.
Candidate genes for the male sterility in D248A.