Table 1.
Prevalence of S. mansoni according to each diagnostic method.
Table 2.
Intensity of infection categories for S. mansoni by each examined diagnostic test.
Table 3.
Sensitivities and specificities of tests in comparison to Kato Katz and LCA.
Fig 1.
shows a negative correlation (Spearman's correlation coefficient = -0.28; p < 0.001) between the intensity of S. mansoni infection determined by POC-CCA test (colour scores) and threshold cycle (Ct) values of S. mansoni specific real-time PCR.
Fig 2.
shows that the number of eggs per gram (EPG) of the KK test correlates positively with the intensity of POC-CCA test band (Spearman's correlation coefficient = 0.57; p < 0.001).
Fig 3.
Threshold cycle (Ct) values of S. mansoni specific real-time PCR correlate negatively with the level of schistosome infection (determined by the Kato Katz method, expressed in eggs per gram (EPG)) (Spearman's correlation coefficient = -0.32; p < 0.001).
S. mansoni real-time PCR-positive samples show a decreasing Ct value with increasing egg count category.
Fig 4.
Sensitivity of Schistosoma POC-CCA urine rapid test and 95% intervals for the diagnosis of S. mansoni infections with low, moderate or high intensities determined by egg counts in stool or threshold cycle (Ct) values of real-time PCR results.
Low intensity infection: KK 1–99 eggs per gram faeces (EPG) or Ct-value of real-time PCR > 30. Moderate intensity infections: KK 100–399 EPG or Ct-value < 30. High intensity infection: KK > 400 EPG and Ct-value of real-time PCR < 30. The specificity of POC-CCA test amounts to 61.5%.