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Fig 1.

Volume rendering reconstructions of the male reproductive system in its anatomical position, in different perspective views.

(a) Right lateral, (b) Left lateral, (c) Dorsal, (d) Ventral.

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Fig 2.

Volume rendering reconstructions of the male reproductive system in different perspective views.

(a, c) Right lateral; (b, d) Dorsal; (e) Testes, lateral-; (f) Testes, dorsal; (g) Testes, postero-anterior; (h) Testes, antero-posterior; (ftl) Fore testicular lobe; (htl) Hind testicular lobe; (lvd) Left vas deferens; (lt) Left testis; (rt) Right testis; (rvd) Right vas deferens.

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Fig 2 Expand

Fig 3.

Abdominal middle-left sagittal section views withAmira Multiplanar slicing.

(a, b, c) Consecutive slices (23 μm thick) from the right towards the left side.

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Fig 3 Expand

Fig 4.

Abdominal Amira Multiplanar sections.

(a, b, c) slices (23 μm thick) according to plane views shown in the right-bottom view.

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Fig 4 Expand

Fig 5.

General configuration, sections, and details of the reproductive structures.

(a) Schematic structure of the male reproductive system, showing the connection between the testes and seminal vesicles (via vasa deferentia) as well as the connection of seminal vesicles and accessory glands to the sperm pump; (b) Dorso-ventral (coronal) section at the level of the accessory glands and seminal vesicles ducts. (c, d, e) Antero-posterior (axial) sections of planes shown with blue, green, and red lines numbered as 1, 2, & 3, respectively; (f) Sagittal section; (g-k) sperm pump and ejaculatory duct (g: right lateral view, h: sagittal section i: ventral view, j: antero-posterior view, k: postero-anterior view); (l-n) Detail of the anterior plate on the sperm pump showing the foramens (l: antero-posterior view, m: postero-anterior view, n: dorsal view).

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Fig 6.

Volume rendering reconstruction of the male abdominal genital terminalia in different perspective views (a-c) and a sagittal right section (d).

(a) Left lateral, (b) Left latero-posterior, (c): Apico-dorsal, (d) Testis, seminal vesicle, accessory gland, sperm pump, ducts, and aedeagus structures superimposed over a sagittal section of the hind abdominal segments.

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Fig 7.

Internal details of the terminalia and aedeagus.

(a) CTVox volume rendering, (b-f) Amira volume renderings, (g) light-microscope right lateral view of the aedeagus, mounted in a slide. Volume renderings with details of the male hind abdominal segments showing the anatomical position of the aedeagus structures and associated musculature. (a, d) right-lateral, (b) postero-anterior, (c) antero-posterior, transparented to display the sclerotized structures of the aedeagus.

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Fig 7 Expand

Fig 8.

Details of the aedeagus in different perspective views.

(a, h) angled left-lateral posterior view, (d) angled right latero-anterior view, (b, e, k) dorsal, (c, g) right-lateral, (i) postero-anterior, (f, j) angled dorsal, (l) ventral. (d-l) For visualization, the renderings have been transparented with Amira software to eliminate soft tissues and enhance harder-sclerotized structures.

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Fig 8 Expand