Fig 1.
Observation of Fusarium verticillioides wild strains and their enhanced green fluorescent protein (EGFP) transformants.
Colony growth (5-day-culture) of each strain. (a) stalk rot wild strain Fv-s, (b) Fv-eGFPs1, an EGFP transformant of Fv-s, (c) Fv-eGFPs2, an EGFP transformant of Fv-s, (d) Fv-eGFPs3, an EGFP transformant of Fv-s, (e) ear rot wild strain Fv-e, (f) Fv-eGFPe1, an EGFP transformant of Fv-e, (g) Fv-eGFPe2, an EGFP transformant of Fv-e, (h) Fv-eGFPe3, an EGFP transformant of Fv-e.
Fig 2.
Detection of target EGFP gene of the strains by PCR amplification.
a,b,c,d) were the transformants from different maize tissues, and e) was the wild type as negative control.
Table 1.
The disease severity index of pathogenicity test between wild-type strains and transformants.
Fig 3.
Hyphae of Fusarium verticillioides transformant spread the infected across cells with a rectangular extension.
Fig 4.
Different degrees of enhanced green fluorescent protein (EGFP)-tagged Fusarium verticillioides infection in stalks of maize.
A) The highest infected internode under the natural light and blue light (Fv-eGFPe1); B) the internode above the ear under the natural light and blue light (Fv-eGFPs1); C) the internode below the ear under the natural light and blue light (Fv-eGFPe1); D and E) the below infected internode under blue light (Fv-eGFPs1).
Fig 5.
Maize ears infected with EGFP-tagged Fusarium verticillioides under blue light.
A) a healthy kernel; B and C), ears infected by Fv-eGFPs1; D, E and F) ears infected by Fv-eGFPe1. Bars indicate 1 mm.
Fig 6.
Infection cycle of maize seeds inoculated with Fusarium verticillioides strains cultured from fluorescent kernels.
A) a healthy maize seed (negative control); B) a seed infected with the re-separated strain of fluorescent kernels; C) discolored seedlings (5-day-growth) with the re-separated strain of fluorescent kernels; D) decayed and discolor radical (5-day-growth) infected with the re-separated strain of fluorescent kernels.