Fig 1.
Measured spectra emitted from the Kr-Cl excimer lamp equipped with a band-pass filter.
Fig 2.
Time-course analysis of DNA damage repair following 254-nm UVC irradiation.
Dorsal skins of mice irradiated with 254-nm UVC at 75 mJ/cm2 were immediately collected post-treatment, as well as 1, 3, 6 and 24 h post-irradiation. (A) CPD-expressing cells were detected by immunohistochemistry (200× magnification). (B) Percentages of CPD-expressing cells per visual field (200× magnification) were enumerated (n = 5).
Fig 3.
Gross appearance of dorsal skin of mice intermittently irradiated with 254-nm or 222-nm UVC.
Dorsal skins of mice subjected to daily irradiation with 254-nm or 222-nm UVC at 450 mJ/cm2/day for a total of 10 days. The gross appearance of the irradiated skin specimens was immediately evaluated after irradiation.
Fig 4.
Histological analysis of dorsal skin of mice intermittently irradiated with 254-nm or 222-nm UVC.
The dorsal skin of mice was subjected to daily sham-irradiation or daily irradiation with 254-nm or 222-nm UVC at 450 mJ/cm2/day for a total of 10 days. At 1 day post-termination of treatment, skin specimens were collected and stained with hematoxylin and eosin (x200 magnification). (A) Epidermal hyperplasia, intracellular edema (square), mitotic figures (red arrow) in the stratum spinosum epidermidis and hyperkeratosis (black arrow) were detected on the dorsal skin of mice irradiated with 254-nm UVC. (B) Skin sections were stained with anti-CPD antibodies (x400 magnification). Arrows indicate CPD-expressing cells.