Fig 1.
Comparison of the fungal and bacterial taxonomic diversity of fecal microbiota of 3 healthy individuals (i1, i2, i3) using 2 storage conditions (within two-hours freezing or RNAlater® dilution before freezing) and 2 extraction protocols (IHMS Protocol Q and PowerSoil® MoBio kit).
Fig 2.
Relative abundance of taxa identified from faeces through 16S-sequencing according to storage or extraction conditions.
Bacterial taxa were identified at genus level from fecal samples of 3 healthy individuals (i1, i2 and i3) using two storage conditions (without additive within two-hours freezing or RNAlater® dilution before freezing) and two extraction protocols (IHMS Protocol Q and PowerSoil® MoBio kit). 16S rRNA gene ultra-deep-sequencing was performed using 454 technology.
Fig 3.
Relative abundance of taxa identified from faeces through ITS-sequencing according to storage or extraction conditions.
Fungal taxa were identified at genus or section level from fecal samples of 2 healthy individuals (i1 and i3) using two storage conditions (without additive within two-hours freezing or RNAlater® dilution before freezing) and two extraction protocols (IHMS Protocol Q and PowerSoil® MoBio kit). ITS1 ultra-deep sequencing was performed using 454 technology.
Fig 4.
Boxplot comparison of log2-abundance of bacterial (A) and fungal (B) taxa according to storage condition. Bacterial diversity was assessed at genus level using 16S rRNA gene ultra-deep sequencing (454 technology) and fungal diversity at genus or section level using ITS1 ultra-deep sequencing. Boxplot of log2-abundance of taxa significantly different (P-value < 0,05) according to storage condition (RNAlater® dilution before freezing [dark blue] vs. without additive within two-hours freezing [light blue]) during analysis at general level are presented.
Fig 5.
Boxplot comparison of log2-abundance of bacterial (A) and fungal (B) taxa according to extraction protocol. Bacterial diversity was assessed at genus level using 16S rRNA gene ultra-deep sequencing (454 technology) and fungal diversity at genus or section level using ITS1 ultra-deep sequencing. Boxplot of log2-abundance of taxa significantly different (P-value < 0,05) according to extraction protocols (PowerSoil® MoBio kit [dark blue] vs. IHMS Protocol Q [light blue]) during analysis at general level are presented.