Table 1.
Relevant clinical data of the liver cohort.
Table 2.
Relevant clinical data of the spleen cohort.
Fig 1.
NK cell phenotype and baseline nutrient receptor expression: Samples were compared using Wilcoxon matched-pairs signed rank tests and multiplicity was controlled for by FDR testing.
Bars indicate the median, significance was defined as p≤0.05 (*). A. viSNE representation of peripheral blood- (PBMC, top row), liver- (middle row) and spleen (bottom row) derived NK cells and their expression of CD56, CD16, CXCR6, CD57 and CD127. B. Expression (Median fluorescence intensity, MdFI) of Glut1 on CD56brightCD16- (purple) and CD56dimCD16+ (teal) tissue-resident (TR), tissue-derived (TD) and peripheral blood (PB) NK cells from paired liver-blood (left diagram, n = 12) and spleen-blood (right diagram, n = 11) samples. C. Expression (MdFI) of CD98 on CD56brightCD16- (purple) and CD56dimCD16+ (teal) tissue-resident (TR), tissue-derived (TD) and peripheral blood (PB) NK cells from paired liver-blood (left diagram, n = 12) and spleen-blood (right diagram, n = 11) samples. D. Expression (MdFI) of CD71 on CD56brightCD16- (purple) and CD56dimCD16+ (teal) tissue-resident (TR), tissue-derived (TD) and peripheral blood (PB) NK cells from paired liver-blood (left diagram, n = 12) and spleen-blood (right diagram, n = 11) samples.
Table 3.
Expression of CXCR6 in NK cells in tissues.
Fig 2.
Effects of cytokine stimulation on Glut1 expression: Samples were compared using Wilcoxon matched-pairs signed rank tests and multiplicity was controlled for by FDR testing.
Bars indicate the median, significance was defined as p≤0.05 (*). A. Representative histograms of Glut1 expression on unstimulated and stimulated CD56bright CXCR6+ (I, grey: unstimulated; purple: stimulated), CD56dim CXCR6+ (II, grey: unstimulated; teal: stimulated), CD56bright CXCR6- (III, grey: unstimulated; purple: stimulated) and CD56dim CXCR6- (IV, grey: unstimulated; teal: stimulated) NK cells from blood (left), liver (middle) and spleen (right) samples. B. Expression (MdFI) of Glut1 on unstimulated (grey) and CD56brightCD16- (purple) and CD56dimCD16+ (teal) tissue-resident (TR), tissue-derived (TD) and peripheral blood (PB) NK cells from paired liver-blood (left diagram, n = 12) and spleen-blood (right diagram, n = 11) samples. C. Differences (fold change) in Glut1 expression (MdFI) between stimulated and unstimulated CD56brightCD16- (purple) and CD56dimCD16+ (teal) tissue-resident (TR), tissue-derived (TD) and peripheral blood (PB) NK cells from paired liver-blood (left diagram, n = 12) and spleen-blood (right diagram, n = 11) samples.
Fig 3.
Effects of cytokine stimulation on CD98 expression: Samples were compared using Wilcoxon matched-pairs signed rank tests and multiplicity was controlled for by FDR testing.
Bars indicate the median, significance was defined as p≤0.05 (*). A. Representative histograms of CD98 expression on unstimulated and stimulated CD56bright CXCR6+ (I, grey: unstimulated; purple: stimulated), CD56dim CXCR6+ (II, grey: unstimulated; teal: stimulated), CD56bright CXCR6- (III, grey: unstimulated; purple: stimulated) and CD56dim CXCR6- (IV, grey: unstimulated; teal: stimulated) NK cells from blood (left), liver (middle) and spleen (right) samples. B. Expression (MdFI) of CD98 on unstimulated (grey) and CD56brightCD16- (purple) and CD56dimCD16+ (teal) tissue-resident (TR), tissue-derived (TD) and peripheral blood (PB) NK cells from paired liver-blood (left diagram, n = 12) and spleen-blood (right diagram, n = 11) samples.
Fig 4.
Effects of cytokine stimulation on CD71 expression: Samples were compared using Wilcoxon matched-pairs signed rank tests and multiplicity was controlled for by FDR testing.
Bars indicate the median, significance was defined as p≤0.05 (*). A. Representative histograms of CD71 expression on unstimulated and stimulated CD56bright CXCR6+ (I, grey: unstimulated; purple: stimulated), CD56dim CXCR6+ (II, grey: unstimulated; teal: stimulated), CD56bright CXCR6- (III, grey: unstimulated; purple: stimulated) and CD56dim CXCR6- (IV, grey: unstimulated; teal: stimulated) NK cells from blood (left), liver (middle) and spleen (right) samples. B. Expression (MdFI) of CD71 on unstimulated (grey) and CD56brightCD16- (purple) and CD56dimCD16+ (teal) tissue-resident (TR), tissue-derived (TD) and peripheral blood (PB) NK cells from paired liver-blood (left diagram, n = 12) and spleen-blood (right diagram, n = 11) samples.