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Fig 1.

CONSORT diagram.

1 As this manuscript was limited to baseline data, there were no withdrawal of participants. 2 To be defined as “sitter” the patient must have a score ≥ 1 on item 9 of the MFM (“with support of one or both upper limbs maintain the seated position for 5 seconds”). For 3 patients the MFM could not be performed at baseline. 2 were classified as sitters since they had a score of 3 at the 6-month follow-up visit and 1 was retrospectively classified as a non-sitter according to patient files. 3 To be defined as “Ambulant” the patient must be able to walk 10 meters without human assistance or use of an ambulation device such as a cane or a walker.

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Table 1.

Baseline clinical and physical characteristics and of enrolled patients.

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Table 1 Expand

Fig 2.

Psychomotor development of patients with SMA type 2 and 3.

A: General motor development; B: Fine motor development; Values for ages displayed on histograms are median (min-max); * p ≤ 0.05.

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Table 2.

Motor function in SMA type 2 and 3 patients.

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Table 3.

Pulmonary function in SMA type 2 and 3 patients.

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Table 4.

Muscle strength in SMA type 2 and 3 patients.

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Table 5.

Electrophysiologic assessment of SMA type 2 and 3 patients.

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Fig 3.

Example of MRI images of patients with SMA type 2 and 3.

A. Example of Dixon images and water T2 map in mildly (upper panel; non-ambulant SMA type 3) and more severely (lower panel; non-sitter SMA type 2) infiltrated patient forearms; B. Example of Dixon images and water T2 map in mildly (upper panel) and more severely (lower panel) infiltrated ambulant patient thighs; C. Example of series of spin echo images at increasing TEs (10 ms steps) in the forearm of a patient and the water T2 map reconstructed from this series using the tri-exp fitting method.

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Table 6.

Muscle MRI imaging of SMA type 2 and 3 patients.

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Table 7.

Molecular SMA biomarkers.

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Fig 4.

Correlation matrix between motor function (MFM) total score and other outcome measures.

Abbreviations: FVC: Forced Vital Capacity; MEP: Maximum Expiratory Pressure; MIP: Maximum Inspiratory Pressure; PCF: Peak Cough Flow; SNIP: Sniff Nasal Inspiratory Pressure; 6MWT: 6-Minutes-Walk Test; 10MWT: 10-Meters-Walk Test; nGyr: norm of the angular velocity of the wrist; nAcc: norm of the acceleration of the wrist; zAcc: vertical acceleration of the wrist; ADM: Abductor Digiti Minimi; CMAP: Compound Muscle Action Potentials; C-CSA: Contractile-Cross Section Area; Abnormal T2: percentage of voxels with abnormal T2 Values in red: Spearman’s correlation test with p ≤ 0.05.

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