Fig 1.
The effect of LY379268 (mGluR2) or NAAG (mGluR3) applied 1 h, 6 h or 24 h after H-I insult and agonists applied together 1 h after H-I on ipsilateral hemisphere weight loss.
The weight deficit was expressed as the percentage of the contralateral (left) hemisphere. Results are presented as the mean values ± SEM, n = 3–5. Statistically significant differences: * p < 0.01.
Fig 2.
Neuroprotective effect of mGluR2 and mGluR3 agonist application observed 7 days after H—I in the CA1 region of the hippocampus and in the cerebral cortex (A). Quantification of surviving neurons in the hippocampal central CA1 region of 100 μm length (B) and in cortex—area 250 μm x 250 μm (C). LY 379268 and NAAG were applied i.p. 1 h and 6 h after H-I. Microphotographs show the hemisphere ipsilateral to H—I.
Fig 3.
The effect of LY379268 and NAAG on the development of apoptosis in the ipsilateral brain hemisphere after H-I: (A) number of TUNEL positive cells detected in the central CA1 area of 100 μm length. (B) number of TUNEL positive cells in the cortex counted in the visual field (250 μm x 250 μm). Number of animals per group n = 3–5. Results are presented as mean values ± SEM. #—different from the sham operated group, p < 0.05; *—different from the H-I group, p < 0.01.
Fig 4.
The effect of LY379268 (A) or NAAG (B) application 1 h or 6 h after H-I on ROS level.
Results are presented as mean ± SEM, n = 3–4; #—different from the sham operated group, p < 0.001; different from H—I group: *- p < 0.005, **—p < 0.002.
Fig 5.
The effect of LY379268 (A) or NAAG (B) application 1 h or 6 h after H-I on changes in SOD activity.
Results are presented as mean ± SEM, n = 4; #—different from sham operated group, p < 0.001; different from H—I group: *—p < 0.01, **—p < 0.005, ***—p < 0.001.
Fig 6.
The effect of LY379268 (A) or NAAG (B) application 1 h or 6 h after H-I on catalase activity.
Results are presented as mean ± SEM, n = 3–4; #—different from sham operated group, p < 0.001, different from H—I group: *—p<0.05, **—p < 0.005, ***—p < 0.001.
Fig 7.
Changes in glutathione peroxidase activity observed after H-I and application of LY379268 (A) or NAAG (B) 1 h or 6 h after the insult.
Results are presented as mean ± SEM, n = 3–4; #—different from sham operated group, p < 0.001; different from H—I group: *—p < 0.05, **—p < 0.001.
Fig 8.
Partial restoration of GSH concentration by LY379268 (A) or NAAG (B) applied at different times after H—I.
Results are presented as mean ± #—SEM, n = 4; #—different from sham operated group, p < 0.001; different from H—I group: *- p < 0.05, **—p < 0.005.