Fig 1.
Schematic overview of sample handling in volunteers (n = 3) and patients (non-small cell lung cancer n = 10, bladder cancer, n = 10).
Fig 2.
Difference in bisulfite modified DNA measured by mean log fold change of ACTB compared to t = 0 after storing urine at room temperature, 4°C, -20°C and -80°C with and without preserving agents.
Error bars represent the standard deviation between three urine samples derived from different donors. RT = room temperature; ACTB = β-actin; EDTA = Ethylenediaminetetraacetic acid.
Fig 3.
DNA derived from urine samples stored at room temperature and 4°C, after 7 days with and without preserving agents.
DNA degradation was measured by the log fold change of ACTB at various conditions compared to day 0. Results of the post-hoc analysis of DNA degradation at room temperature are presented above and under the small bars. EDTA = Ethylenediaminetetraacetic acid; PenStrep = Penicillin Streptomycin.
Fig 4.
Scatter plots showing ΔCT of β actin (ACTB) and the ΔCT of methylated RASSF1A with or without various preserving agents (preservatives) at room temperature and at 4°C after 7 days of storage.
The outliers are marked with A-E.
Table 1.
The difference between the ΔCTACTB and ΔCTRASSF1A (day 7) for various conditions.