Table 1.
Spikelet fertility (SF) of 84 introgression lines (ILs) under cold water irrigation selected from 5 Chaoyou 1 (CY1, the recipient) BC2 populations in 2008, 2009 and 2010.
Fig 1.
The population development for identification and validation of QTL for cold tolerance in rice.
Table 2.
Summary statistics of five random backcross populations (BC2F6) for spikelet fertility (in %) evaluated under the cold water stress and normal control conditions in 2010.
Fig 2.
Wald tests of single and combined five populations selected for spikelet fertility under cold water stress: a): population CY1/X22 (A); b): population CY1/Yuanjing7 (B); c): population CY1/ Fengaizhan (C); d): population CY1/ Chhomrong (D); e): population CY1/ Doddi (E); f): population combined five populations. The horizontal broken line indicated that Wald value is 28.0 threshold at P value of 0.01.
Table 3.
Quantitative trait loci (QTL) for rice cold tolerance (CT) detected in five selected introgression populations using single and joint segregation distortion analyses.
Table 4.
Detection of QTL, via t-tests, affecting cold tolerance associated with spikelet fertility (%) under cold water in five random BC2F4 populations.
Fig 3.
Genomic regions harboring FGUs and QTLs underlying rice cold tolerance at the reproductive stage detected in 84 introgression lines (ILs) from 5 BC2F4 populations (Tables 2 and 4), in which regions pointed by purple arrows are QTLs detected in the random populations.
Table 5.
Genomic information for 46 functional genetic units (FGUs) (37 single loci and 9 association groups or AGs) for cold tolerance (CT) detected by χ2 tests (single loci) and multi-locus linkage disequilibrium analyses in 84 cold-tolerant introgression lines (ILs) selected from five populations.
Fig 4.
Putative genetic networks (multi-locus structures) underlying cold tolerance (CT) of rice detected in HT backcross introgression lines (BILs) from five (A, B, C, D, E and F) populations. In the corresponding graphical genotypes of each network, the unfilled, fully colored, and patched cells represent the recipient homozygote, donor homozygote, and heterozygote genotypes. The numbers in the cells of each FGU are the number of loci included in the FGUs. The loci (markers) included in each of the detected association groups (AGs) are shown in Table S1. Solid arrow lines connected two FGUs in each branch of a network represent putative functional relationships with those of high introgression as putative regulators in the upstream and those of low introgression in the downstream, and the thickness of an arrow line was proportional to the introgression frequency of the downstream FGU in Table 5.