Table 1.
Obtained isolates of Paecilomyces.
Fig 1.
(A) Creatine sucrose agar (CREA) plate inoculated with isolate Pf623 which was unable to grow and produce acid, hence an unchanged purple plate color and (B) CREA plate inoculated with isolate Pf613 which was able to grow and produce acid at 25°C for seven days in continuous dark condition; acid production indicated by the color change to yellow.
Table 2.
Source of used isolates in phylogeny.
Fig 2.
Most parsimonous tree based on combined data set of ITS, β-tubulin and calmodulin sequences.
sequences are from 18 selected isolates and close taxa using MEGA 7 software, showing the relationship among our isolates and Paecilomyces formosus isolates, especialy to P. lecythidis. The percentages above and below the branches are the frequencies of a given branch appeared in 1000 bootstrap replications. Bootstrap values less than 50% are not displayed. Thermoascus crustaceus has been used as outgroup.
Fig 3.
Most parsimonous tree based on combined data set of ITS, β-tubulin and calmodulin sequences.
sequences are from 62 selected isolates and close taxa using MEGA 7 software, showing low variation among our isolates. Isolates from Pistacia vera, Isolates from Pistacia mutica, Isolates from Punica granatum, Isolates from Tamarix hispida, Isolates from Tamarix aphylla, Isolates from Prunus amygdalus, Isolates from Haloxylon sp., Isolates from Nerium oleander, Isolates from Caesalpinia gilliesii and Isolates from the air of pistachio orchards. The percentages above and below the branches are the frequencies of a given branch appeared in 1000 bootstrap replications. Bootstrap values less than 50% are not displayed. Paecilomyces formosus CBS990.73B has been used as outgroup.
Table 3.
Strains and their sequences from GenBank (NCBI) used in phylogenetic analysis.
Fig 4.
(A) Inoculation of P. formosus on two years old twigs of pistachio in orchard. Fungus development and canker formation are evident. The fungus develops along the axis of the branch. (B) Control inoculation and callus formation in pathogen free scar.