Fig 1.
Maximum Likelihood tree of the 16S rRNA gene of Cuspidothrix issatschenkoi.
Strains from this study are marked in bold. Bootstrap values above 50 are included. The scale bar indicates 0.5% sequence divergence.
Table 1.
Similarities of the 16S rRNA gene based on 1,063 base pairs for Cuspidothrix issatschenkoi strain NIVA-CYA 711 (FN689797; Germany), CAWBG02 (EF638715; New Zealand), CHABD3 (FJ424558; China), LBRI48 (KM245026; Japan) and RM-6 (KM245027; Japan).
The values in the upper right triangle are the percent identities between a pair of sequences. The values in the lower left triangle are the numbers of dissimilar nucleotides.
Table 2.
Similarities of the ana gene cluster (anaA-anaG) based on 22,802 base pairs for Cuspidothrix issatschenkoi strain NIVA-CYA 711 (LT984882; Germany), CAWBG02 (LT984883; New Zealand), CHABD3 (KM245025; China): LBRI48 (KM245023; Japan) and RM-6 (KM245024; Japan).
The values in the upper right triangle are the percent identities between a pair of sequences. The values in the lower left triangle are the numbers of dissimilar nucleotides.
Fig 2.
Maximum Likelihood tree based on the ana gene clusters of five Cuspidothrix issatschenkoi strains included in this study.
Bootstrap values above 50 are included. The scale bar indicates 0.1% sequence divergence.
Fig 3.
Maximum Likelihood tree based on the anaF gene clusters of the five Cuspidothrix issatschenkoi strains included in this study.
anaF genes of Anabaena sp., Oscillatoria sp., Cylindrospermum stagnale are included in the tree. A homologous sequence of Moorea producens is used as outgroup. Bootstrap values above 50 are included. The scale bar indicates 2% sequence divergence.
Table 3.
Genes and non-coding regions in the ana gene cluster with single nucleotide polymorphisms (SNP) compared to Cuspidothrix issatschenkoi strain CHABD3 from China.
Tn = Transition, Tv = Transversion, In = Insertion, Del = Deletion. CAWBG02 (New Zealand), NIVA-CYA 711 (Germany), RM-6 (Japan), LBRI48 (Japan).