Table 1.
Sequences of primers and probes used for qRT-PCR.
Table 2.
Sample characterization.
Fig 1.
AR-Vs mRNA expression in BPH and PCa samples.
All mRNA data are normalized by beta-2-microglobulin (B2M). For AR-FL, AR-V1 and AR-V4, values of normalized quantity means (NQM) were used to create the graphs. AR-V7rv was obtained from the ratio between AR-V4V7 and AR-V4. The bars represent the minimum to maximum values. (A) The median AR-FL expression is not significantly different comparing BPH and PCa (P = 0.331). (B) and (D) AR-V1 and AR-V7rv expression are higher in primary PCa than in BPH (P = 0.041 and P<0.001, respectively). (C) AR-V4 expression is significantly different comparing BPH and PCa (P = 0.001), showing higher levels in hyperplastic tissue. P value was determined by the Mann–Whitney U test.
Table 3.
Cell cycle-related and apoptosis-related genes mRNA expression.
Fig 2.
Histological score for immunohistochemical staining of AR-NTD, AR-CTD, and AR-V7.
A variety of immunohistochemical staining patterns for AR-NTD, AR-CTD and AR-V7 are shown. Epithelial nuclear staining (A) for AR-NTD (PG21) and AR-CTD (C19) are higher in BPH samples than in PCa samples (P = 0.034 and P = 0.041, respectively). Although BPH group seems to have a higher AR-V7 H-Score, it was not statistically different (P = 0.093). Epithelial cytoplasmatic staining (B) was not different between the groups. The bars represent the minimum to maximum values.
Fig 3.
Representative images of IHC staining.
100X and 400X. For H-Score calculation we analyzed all over the slides.
Table 4.
Histological scores (H-Score) representing the histological staining patterns of AR-NTD, AR-CTD and AR-V7 protein in BPH and PCa samples.
Table 5.
Correlation coefficients between AR-Vs and clinical features.
Table 6.
Correlation coefficients between cell cycle-related and apoptosis-related genes and other variables.
Fig 4.
Stratification of PCa samples between Gleason grade (≤7(3+4) and ≥7(4+3).
(A) Age at surgery. The group low grade (≤7(3+4) was younger than the high grade group (≥7(4+3) (63 and 68 years, respectively. P = 0.047). (B) AR-FL levels were similar between groups (P = 0.836). (C) AR-V1 levels failed to reach significance (P = 0.060), but seems to be up-regulated in high grade group (≥7(4+3). (D) and (E) AR-V4 and AR-V7rv levels are higher in high grade group (≥7(4+3) (P = 0.004 and P = 0.014). (F) AR-FL/AR-V4 ratio was higher in low grade group (≤7(3+4), P = 0.021). The bars represent the minimum to maximum values.