Table 1.
Group C orthobunyavirus (GRCV) reference strains used in the study.
Table 2.
Primers used in RT-PCR and sequencing for group C orthobunyavirus (GRCV).
Fig 1.
Distribution of febrile patient samples in Peru.
Number of febrile surveillance samples (denominator) and number of group C isolates (numerator). The green circles represent the places where group C viruses were isolated and the size of the circle is proportional to the number of isolates.
Fig 2.
Phylogenetic analysis of group C viruses based on partial nucleotide sequences of the S segment.
The phylogenetic tree was constructed using the neighbor-joining method. Bootstrap values were obtained based on 1000 replicates on the bases of partial coding sequence of N and NSs protein. The evolutionary distances were computed using the Kimura 2-parameter method. Members of the Simbu, California and Bunyamwera serogroups were used as outgroups to root the tree.
Table 3.
Nucleotide and amino acid sequence identity values for a partial sequence of the S segment from some isolates and reference group C orthobunyaviruses.
Fig 3.
Phylogenetic analysis of group C viruses based on partial nucleotide sequences of the M segment.
The phylogenetic tree was constructed using the neighbor-joining method. Bootstrap values were obtained based on 1000 replicates using the partial coding sequence of the polyprotein. The evolutionary distances were computed using the Kimura 2-parameter method. Members of the Simbu, California and Bunyamwera serogroups were used as outgroups to root the tree.
Table 4.
Nucleotide and amino acid sequence identity values for partial sequence of the M segment from some isolates and reference group C orthobunyaviruses.
Fig 4.
Phylogenetic analysis of group C viruses based on partial nucleotide sequences of the L segment.
The phylogenetic tree was constructed using the neighbor-joining method. Bootstrap values were obtained based on 1000 replicates on the bases of partial coding sequence of RdRp. The evolutionary distances were computed using the Kimura 2-parameter method. Members of the Simbu, California and Bunyamwera serogroups were used as outgroups to root the tree.
Table 5.
Nucleotide and amino acid sequence identity values for a partial sequence of the L segment of some isolations and referential group C orthobunyavirus.
Table 6.
Microneutralization test of group C orthobunyavirus (GRCV) using hyperimmune mouse ascitic fluids (HMAFs) prepared with prototype strains.