Fig 1.
Timeline of the transplantation procedure, blood sampling and administration of heparin.
T1: induction of anaesthesia; T2: 5 minutes after reperfusion of the kidney; T3: 2 hours post-operative.
Table 1.
Baseline characteristics and intraoperative parameters.
Data are given as mean (SD) and median (IQR) or n (%). Fishers’ Exact test or Chi-squared test were used in case of categorical data. Continuous data were tested with ANOVA/ Kruskal-Wallis in case of three groups and with student t-test/Mann-Whitney in case of 2 groups.
Fig 2.
Markers of platelet activation.
Fig 2A: Levels of platelet factor 4. Preemptive group (blue triangle), non-preemptive group (red square) and donors (green dot) before incision (T1), 5 minutes after reperfusion (T2) and 2 hours after surgery (T3). Data are given as medians with IQR. Fig 2B: Levels of soluble P-selectin. Preemptive group (blue triangle), non-preemptive group (red square) and donors (green dot) before incision (T1), 5 minutes after reperfusion (T2) and 2 hours after surgery (T3). Data are given as medians with IQR.
Fig 3.
Markers of coagulation activation.
Fig 3A: Levels of prothrombin fragment 1+2. Preemptive group (blue triangle), non-preemptive group (red square) and donors (green dot) before incision (T1), 5 minutes after reperfusion (T2) and 2 hours after surgery (T3). Data are given as medians with IQR. Fig 3B: Levels of D-dimer. Preemptive group (blue triangle), non-preemptive group (red square) and donors (green dot) before incision (T1), 5 minutes after reperfusion (T2) and 2 hours after surgery (T3). Data are given as medians with IQR.
Fig 4.
Preemptive group (blue triangle), non-preemptive group (red square) and donors (green dot) before incision (T1), 5 minutes after reperfusion (T2) and 2 hours after surgery (T3). Data are given as medians with IQR.
Fig 5.
Fig 5A. TGA lagtime (A). Preemptive group (blue triangle), non-preemptive group (red square) and donors (green dot) before incision (T1) and 2 hours after surgery (T3). Data are given as medians with IQR. Fig 5B. TGA peak. Preemptive group (blue triangle), non-preemptive group (red square) and donors (green dot) before incision (T1) and 2 hours after surgery (T3). Data are given as medians with IQR. Fig 5C. TGA endogenous thrombin potential (ETP). Preemptive group (blue triangle), non-preemptive group (red square) and donors (green dot) before incision (T1) and 2 hours after surgery (T3). Data are given as medians with IQR. Fig 5D: TGA velocity index. Preemptive group (blue triangle), non-preemptive group (red square) and donors (green dot) before incision (T1) and 2 hours after surgery (T3). Data are given as medians with IQR.
Fig 6.
Preemptive group (blue triangle), non-preemptive group (red square) and donors (green dot) before incision (T1) and 2 hours after surgery (T3). Data are given as medians with IQR.
Fig 7.
Martius Scarlet Blue stained reperfusion biopsies.
Fibrin stains red, collagen blue. Fig 7A reperfusion biopsy negative for fibrin. Fig 7B reperfusion biopsy positive for fibrin Fig 7C reperfusion biopsy positive for fibrin Fig 7D reperfusion biopsy positive for fibrin.